| 基于响应面法优化莱氏绿僵菌SZCY200812的液相发酵条件 |
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| 引用本文： | 薛锐,付玉飞,邓细周,沈绍运,杜广祖,陈斌.基于响应面法优化莱氏绿僵菌SZCY200812的液相发酵条件[J].中国生物防治学报,2022,38(6):1484-1494. |
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| 作者姓名： | 薛锐 付玉飞 邓细周 沈绍运 杜广祖 陈斌 |
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| 作者单位： | 云南农业大学植物保护学院/云南生物资源保护与利用国家重点实验室, 昆明 650201 |
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| 基金项目： | 云南省重点领域科技计划（202001BB050002）；国家重点研发计划（2019YFD0300105） |
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| 摘 要： | 为提高莱氏绿僵菌菌株SZCY200812液相发酵时孢子萌发率和生物量，为固相发酵产孢提供高质发酵液，本研究采用单因素及Box-Behnken试验设计结合的方法对该菌液相发酵过程中培养液种类、装液量、pH、摇床转速及培养温度等条件进行优化，确定最佳的液相发酵培养条件。结果表明，通过单因素试验初步确定利用SDY培养液、装液量100/250mL、pH7、摇床转速180r/min及温度25℃，第120h每10mL液态发酵生物产量为0.8433g。通过响应面法建立了莱氏绿僵菌SZCY200812液体发酵培养菌丝生物量与自变量回归模型，根据模型所得结果得出装液量为100.82/250mL，摇床转速178.85r/min，温度24.80℃，pH6.73条件下为理论最佳液态发酵培养条件。为便于生产，得出该菌株SZCY200812在SDY培养液中最佳的培养条件为：装液量101/250mL、pH6.7、摇床转速179r/min以及温度25℃，第120h每10mL液相发酵生物产量达0.8637g。综合以上结果，影响莱氏绿僵菌SZCY200812液相发酵的因素由强至弱依次为pH（D）、温度（C）、摇床转速（B）和装液量（A）。
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| 关 键 词： | 莱氏绿僵菌 液相发酵 培养条件优化 响应面法 |
| 收稿时间： | 2021-11-02 |
Optimization of Liquid-state Fermentation Conditions of Metarhizium rileyi SZCY200812 by Response Surface Methodology |
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| XUE Rui,FU Yufei,DENG Xizhou,SHEN Shaoyun,DU Guangzu,CHEN Bin.Optimization of Liquid-state Fermentation Conditions of Metarhizium rileyi SZCY200812 by Response Surface Methodology[J].Chinese Journal of Biological Control,2022,38(6):1484-1494. |
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| Authors: | XUE Rui FU Yufei DENG Xizhou SHEN Shaoyun DU Guangzu CHEN Bin |
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| Institution: | College of Plant Protection, Yunnan Agricultural University/State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Kunming 650201, China |
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| Abstract: | It is important to increase the liquid fermentation germination rate and biomass of Metarhizium rileyi strain SZCY200812 and provide high quality fermentation broth for solid phase fermentation. The best liquid fermentation conditions were determined in this study using the single factor and Box-Behnken experimental design to optimize the culture medium, liquid volume, pH, rotating speed, and culture temperature in the liquid fermentation process. The results showed a biomass yield of 0.8433 g per 10 mL at 120 h of fermentation employing conditions of SDY culture medium, liquid volume of 100/250 mL, pH of 7, rotating speed of 180 r/min and temperature of 25 ℃. The regression models of mycelial biomass in liquid fermentation culture of M. rileyi SZCY200812 with independent variables were established by response surface methodology. According to the results of the model, the optimal liquid fermentation conditions were liquid volume of 100.82/250 mL, rotation speed of 178.85 r/min, temperature of 24.80 ℃, and pH 6.73, which produced a biomass of liquid phase fermentation of 0.8637 g per 10 mL at 120 h. The RSM analysis also indicated that the influence on biomass from high to low were pH, temperature, rotating speed, liquid volume. |
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| Keywords: | Metarhizium rileyi liquid-state fermentation optimization of culture conditions response surface methodology |
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