| 环境条件对Bacillus altitudinis LZP02生物膜形成的影响 |
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| 引用本文： | 黄东慧,钟鹏,王建丽,胡云龙,王志刚.环境条件对Bacillus altitudinis LZP02生物膜形成的影响[J].浙江农业学报,2022,34(7):1466. |
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| 作者姓名： | 黄东慧 钟鹏 王建丽 胡云龙 王志刚 |
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| 作者单位： | 1.齐齐哈尔大学 生命科学与农林学院,黑龙江 齐齐哈尔 1610062.黑龙江省农业微生物制剂产业化技术创新中心,黑龙江 齐齐哈尔 1610063.黑龙江省农业科学院 畜牧兽医分院,黑龙江 齐齐哈尔 1610054.黑龙江省农业科学院 草业研究所,黑龙江 哈尔滨 150086 |
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| 基金项目： | 国家自然科学基金(31870493);黑龙江省重点研发计划(GA21B007);齐齐哈尔大学研究生创新项目(YJSCX2020042) |
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| 摘 要： | Bacillus altitudinis LZP02是一株水稻根际促生菌,可在水稻根际定殖,形成生物膜。为研究Bacillus altitudinis LZP02生物膜的形成情况,在不同培养条件下,通过96孔微量平板法观察生物膜形成,并采用结晶紫染色法对其进行定量。结果表明,B. altitudinis LZP02在培养基表面形成稳定的生物膜结构,具有良好的生物膜形成能力。其形成生物膜的最适温度为30 ℃,在25 ℃只形成少量生物膜,说明低温降低了B. altitudinis LZP02生物膜形成能力;在pH值为4~10时均能形成生物膜,pH值为7.0时生物膜形成能力最强。Ca2+对B. altitudinis LZP02生物膜形成没有明显的影响,Fe2+、Fe3+、Mg2+、K+、Na+、Zn2+、Cu2+可以抑制B. altitudinis LZP02生物膜的形成,Mn2+能够促进B. altitudinis LZP02生物膜的形成。该研究结果为B. altitudinis LZP02的进一步开发利用提供了重要参考,也为深入探究B. altitudinis LZP02生物膜形成机制提供了现实依据。
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| 关 键 词： | Bacillus altitudinis LZP02 生物膜 金属离子 水稻根际促生菌 |
| 收稿时间： | 2021-10-11 |
Effects of environmental conditions on biofilm formation of Bacillus altitudinis LZP02 |
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| HUANG Donghui,ZHONG Peng,WANG Jianli,HU Yunlong,WANG Zhigang.Effects of environmental conditions on biofilm formation of Bacillus altitudinis LZP02[J].Acta Agriculturae Zhejiangensis,2022,34(7):1466. |
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| Authors: | HUANG Donghui ZHONG Peng WANG Jianli HU Yunlong WANG Zhigang |
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| Abstract: | Bacillus altitudinis LZP02 is a rice rhizosphere growth-promoting bacterium, which can colonize rice rhizosphere to form biofilm. In order to study the formation of B. altitudinis LZP02 biofilm under different culture conditions, the formation of B. altitudinis LZP02 biofilm was observed by 96-well microplate method, and it was quantified by crystal violet staining. The results showed that B. altitudinis LZP02 formed a stable biofilm structure on surface of the culture medium and had good biofilm formation ability.The optimum temperature of B. altitudinis LZP02 for biofilm formation was 30 ℃, but only a few biofilms were formed at 25 ℃, which indicated that low temperature reduced the biofilm formation ability of B. altitudinis LZP02.Biofilm could be formed at pH 4-10, and the ability of biofilm formation was strongest at pH 7.0. Ca2+ had no obvious effect on the formation of B. altitudinis LZP02 biofilm. Fe2+, Fe3+, Mg2+, K+, Na+, Zn2+ and Cu2+ inhibited the formation of B. altitudinis LZP02 biofilm, while Mn2+ promoted the formation of B. altitudinis LZP02 biofilm.The research results provided an important reference for the further development and utilization of B. altitudinis LZP02, and also provided a realistic basis for further exploring the biofilm formation mechanism of B. altitudinis LZP02. |
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| Keywords: | Bacillus altitudinis LZP02 biofilm metal ions rhizosphere growth-promoting bacterium of rice |
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