| NAC通过抑制大鼠肺细胞凋亡缓解镉致肺组织损伤 |
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| 引用本文： | 仝锡帅,余耿生,李雅文,冉迪,顾建红,邹辉,袁燕,卞建春,刘宗平.NAC通过抑制大鼠肺细胞凋亡缓解镉致肺组织损伤[J].畜牧兽医学报,2022,53(9):3241-3250. |
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| 作者姓名： | 仝锡帅 余耿生 李雅文 冉迪 顾建红 邹辉 袁燕 卞建春 刘宗平 |
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| 作者单位： | 1. 扬州大学农业科技发展研究院(教育部农业与农产品安全国际联合研究实验室), 扬州 225009;2. 扬州大学兽医学院, 扬州 225009;3. 江苏省动物重要疫病与人兽共患病防控协同创新中心, 扬州 225009 |
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| 基金项目： | 国家自然科学基金（32102732；31872533）；江苏省自然科学基金资助项目（BK20210806）；江苏高校优势学科建设工程资助项目（PAPD） |
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| 摘 要： | 本试验旨在探讨N-乙酰半胱氨酸（NAC）在镉（Cd）致去卵巢大鼠肺细胞凋亡和肺组织损伤的缓解作用。40只雌性SD大鼠随机分为假手术对照（Control）组、Cd组、NAC组和NAC+Cd组，每组10只。18个月后，取大鼠肺组织，镜检观察组织HE染色与Masson染色后的病理学变化，应用火焰原子吸收光谱法检测组织中Cd的含量，应用qRT-PCR法检测组织纤维化关键蛋白Col-I和Col-III mRNA水平，并应用Western blot检测凋亡关键蛋白（Bcl-2、Bax及cleaved caspase-3）及p53的表达与Akt磷酸化。结果显示，与Control组相比，Cd组肺组织中肺泡结构消失和纤维组织增生，Cd的含量极显著增加（P<0.01），Col-I和Col-III mRNA水平、Bax/Bcl-2比值、cleaved caspase-3与p53的表达极显著上调（P<0.01），Akt磷酸化受抑制。与Cd组相比，NAC+Cd组减轻了肺组织病理变化，显著（P<0.01）降低了肺组织中Cd的含量，下调了Col-I和Col-III mRNA水平和Bax/Bcl-2比值、cleaved caspase-3与p53的表达，提高了Akt磷酸化水平。综上表明，NAC对Cd致大鼠肺组织损伤和肺细胞凋亡具有较好的缓解作用。
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| 关 键 词： | 镉(Cd) N-乙酰半胱氨酸(NAC) 肺组织 大鼠 凋亡 |
| 收稿时间： | 2021-12-21 |
NAC Alleviates Cadmium-induced Lung Damage by Inhibiting the Apoptosis of Lung Cell in Rats |
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| TONG Xishuai,YU Gengsheng,LI Yawen,RAN Di,GU Jianhong,ZOU Hui,YUAN Yan,BIAN Jianchun,LIU Zongping.NAC Alleviates Cadmium-induced Lung Damage by Inhibiting the Apoptosis of Lung Cell in Rats[J].Acta Veterinaria et Zootechnica Sinica,2022,53(9):3241-3250. |
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| Authors: | TONG Xishuai YU Gengsheng LI Yawen RAN Di GU Jianhong ZOU Hui YUAN Yan BIAN Jianchun LIU Zongping |
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| Institution: | 1. Institutes of Agricultural Science and Technology Development (Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China), Yangzhou University, Yangzhou 225009, China;2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China |
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| Abstract: | The aim of this study was to evaluate the alleviation effect of N-acetyl-L-cysteine (NAC) on cadmium (Cd) exposure-induced the apoptosis of lung cells and the damage of lung tissue in the ovariectomized rats. Forty adult female Sprague-Dawley (SD) rats were randomly divided into sham operation group (Control), Cd group, NAC group (NAC), and NAC+Cd group (NAC+Cd). Each group contained 10 rats. After feeding for 18 months, the lung tissue of the rat was separated for further tests. The pathological change of lung tissue was observed after the HE staining and the Masson staining. The content of Cd in lung tissue was detected by atomic absorption analyzer. The mRNA levels of lung tissue fibrotic key proteins (Col-I and Col-III) were analyzed by the qRT-PCR. In addition, the expression of key apoptosis proteins (Bcl-2, Bax, and cleaved caspase-3) and p53, and Akt phosphorylation were evaluated by Western blot. The results showed that the disappearance of alveolar structure and the hyperplasia of fibrous tissue, the content of Cd was significantly (P<0.01) increased, the mRNA levels of Col-I and Col-III were significantly (P<0.01) up-regulated, and the ratio of Bax/Bcl-2, the expression of cleaved caspase-3 and p53 were increased in lung tissue of Cd group compared with Control group, but the phosphorylation of Akt was inhibited. The pathological change, the content of Cd, the mRNA levels of Col-I and Col-III, and the ratio of Bax/Bcl-2, the expression of cleaved caspase-3 and p53 were decreased in lung tissue of NAC+Cd group compared with Cd group, but the phosphorylation of Akt was increased. These results indicated that NAC has a certain alleviating effect on Cd-induced the damage of lung tissue and the apoptosis of lung cells in rats. |
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| Keywords: | cadmium (Cd) N-acetyl-L-cysteine (NAC) lung tissue rats apoptosis |
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