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| 基于SSR-HRM技术的向日葵DNA指纹图谱构建 | |
| 引用本文： | 刘苡含,牟青山,陈珊宇,阮关海,胡晋,关亚静.基于SSR-HRM技术的向日葵DNA指纹图谱构建[J].浙江农业学报,2022,34(4):678-686. |
| 作者姓名： | 刘苡含 牟青山 陈珊宇 阮关海 胡晋 关亚静 |
| 作者单位： | 1.浙江大学 农业与生物技术学院,浙江 杭州 3100582.海南浙江大学研究院,海南 三亚 5720253.浙江省农业科学院 作物与核技术利用研究所,浙江 杭州 310021 |
| 基金项目： | 浙江省重点研发计划(2019C02004) |
| 摘 要： | 以浙江省各地种植的32份向日葵种质为材料,利用17对简单重复序列标记引物结合高分辨率熔解曲线技术对32份材料进行遗传多样性分析与DNA指纹图谱构建.聚类分析结果表明,该32份材料遗传多样性相对较低,大部分材料的亲缘关系与其地理来源、所属类型基本一致.本研究创新了向日葵种质资源的鉴定方法,为进一步利用高分辨率熔解曲线技术... |
| 关 键 词： | 向日葵 SSR分子标记 高分辨率熔解曲线 指纹图谱 遗传多样性 |
| 收稿时间： | 2021-06-23 |
Establishment of DNA fingerprint for sunflower by SSR-HRM technique |
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| LIU Yihan,MOU Qingshan,CHEN Shanyu,RUAN Guanhai,HU Jin,GUAN Yajing.Establishment of DNA fingerprint for sunflower by SSR-HRM technique[J].Acta Agriculturae Zhejiangensis,2022,34(4):678-686. | |
| Authors: | LIU Yihan MOU Qingshan CHEN Shanyu RUAN Guanhai HU Jin GUAN Yajing |
| Institution: | 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China 2. Hainan Institute, Zhejiang University, Sanya 572025,Hainan,China 3. Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
| Abstract: | Combined with high-resolution melting curve analysis technology, 17 pairs of core simple sequence repeats (SSR) primers were screened out for genetic diversity analysis and DNA fingerprint construction of 32 sunflower lines collected from Zhejiang Province.The results of cluster analysis showed that genetic diversity among the 32 materials was relatively low, and genetic relationships among most of 32 materials were consistent with their geographical sources and types. This study innovated the identification method of sunflower germplasm resources, and laid a foundation for further research on identification of sunflower germplasm by microsatellite high-resolution melting technique. |
| Keywords: | sunflower SSR markers high-resolution melting curve fingerprint map genetic diversity |
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