| 玉米转录因子bZIP G亚家族基因的表达模式 |
| |
| 引用本文： | 贾利强,赵秋芳,陈曙,丁波.玉米转录因子bZIP G亚家族基因的表达模式[J].浙江农业学报,2022,34(2):221. |
| |
| 作者姓名： | 贾利强 赵秋芳 陈曙 丁波 |
| |
| 作者单位： | 1.贵州师范学院 生物科学学院,贵州 贵阳 5500182.中国热带农业科学院 南亚热带作物研究所,广东 湛江 524091 |
| |
| 基金项目： | 贵州师范学院博士项目(2019BS004) |
| |
| 摘 要： | 为探究不同逆境胁迫(盐、干旱、温度和硝态氮/铵态氮缺乏胁迫)对玉米ZmbZIP基因表达模式的影响,以玉米骨干自交系郑58为实验材料,设置200 mmol·L-1 NaCl、20% PEG6000、4 ℃低温、硝态氮或铵态氮缺乏胁迫试验,探测其对bZIP家族基因G亚家族成员表达模式的影响。进化树分析结果显示,G亚家族成员进一步细分为3个亚组。qRT-PCR分析结果表明,15个ZmbZIPs基因具有不同的组织表达模式,表明这些基因在玉米生长发育中发挥不同的作用。人为模拟盐、干旱、低温和氮缺乏胁迫实验中,20个ZmbZIPs基因受不同逆境因子的广泛调控,预示ZmbZIP广泛参与调控玉米应答逆境胁迫响应途径。
|
| 关 键 词： | bZIP转录因子 逆境胁迫 基因表达 玉米 |
| 收稿时间： | 2021-05-19 |
Expression analysis of bZIP G subfamily genes in maize |
| |
| JIA Liqiang,ZHAO Qiufang,CHEN Shu,DING Bo.Expression analysis of bZIP G subfamily genes in maize[J].Acta Agriculturae Zhejiangensis,2022,34(2):221. |
| |
| Authors: | JIA Liqiang ZHAO Qiufang CHEN Shu DING Bo |
| |
| Institution: | 1. School of Biological Sciences, Guizhou Education University, Guiyang 550018, China
2. South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, Guangdong, China |
| |
| Abstract: | To explore the effect of various abiotic stresses on expression pattern of ZmbZIP, maize inbreed line Zheng58 was adopted as test materials, and five abiotic stresses (200 mmol·L -1 NaCl, 20% PEG6000, 4 ℃ low temperature, nitrate or ammonium deficiency stresses) were set, and expression of G group genes of bZIP family were systematically detected. Phylogenetic tree analysis showed that G group genes were subdivided into 3 subgroups. qRT-PCR analysis showed that 15 ZmbZIP genes had different expression pattern in various maize tissues, indicating their important roles during growth and development process in maize. Under different stresses of simulated salt, drought, low temperature and nitrogen deficiency, 20 ZmbZIP genes were widely regulated, indicating that most of those genes were involved in response to stress signaling pathway. |
| |
| Keywords: | |
|
| 点击此处可从《浙江农业学报》浏览原始摘要信息 |
| 点击此处可从《浙江农业学报》下载免费的PDF全文 |
|
