| 低能离子注入凤仙花后DNA变异的RAPD分析 |
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| 引用本文： | 孙永健,孙宁,张乃楠,曹智攀,张磊,陈小强.低能离子注入凤仙花后DNA变异的RAPD分析[J].天津农学院学报,2009,16(1):21-24. |
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| 作者姓名： | 孙永健 孙宁 张乃楠 曹智攀 张磊 陈小强 |
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| 作者单位： | 1. 天津农学院,农学系,天津,300384
2. 天津大学,化工学院,天津,300072 |
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| 基金项目： | 天津市农业科技发展计划 |
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| 摘 要： | 将3种不同剂量的低能P＋和N＋注入来自同一纯系品种的风仙花（Impatiens balsamina L．）种子中后，采用RAID技术研究风仙花的变异。首先，建立了凤仙花的RAPD反应体系，然后，从25个随机引物中筛选出扩增条带清晰、反应结果稳定、重复性较好的3个引物，共扩增出86条较清晰的谱带，其中多态性谱带23条，分子量在350-2000bp之间。通过分析DNA的变异情况发现，注入低能离子可以使凤仙花发生DNA水平上的变异。这一研究为花卉产业的发展提供了一种新的育种手段。
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| 关 键 词： | 离子注入 风仙花 随机扩增多态性DNA |
RAPD Analysis of Impatiens balsamina L. DNA Variation Caused by Injection of Low Energy Ions |
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| SUN Yong-jian,SUN Ning,ZHANG Nai-nan,CAO Zhi-pan,ZHANG Lei,CHEN Xiao-qiang.RAPD Analysis of Impatiens balsamina L. DNA Variation Caused by Injection of Low Energy Ions[J].Journal of Tianjin Agricultural College,2009,16(1):21-24. |
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| Authors: | SUN Yong-jian SUN Ning ZHANG Nai-nan CAO Zhi-pan ZHANG Lei CHEN Xiao-qiang |
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| Institution: | 1. Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China; 2. College of Chemical Engineering, Tianjin University, Tianjin 300072, China) |
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| Abstract: | Low energy P＋ and N＋ ions were implanted to Impatiens balsamina L. seeds, and the best RAPD （random amplified polymorphic DNA） reaction system for Impatiens balsamina L. was established. By using random primers, DNA variation was analyzed. The results show that 3 primers were the most efficient among 25 primers, and 23 polymorphic bands were found among 86 bands gained by RAPD. The results suggest that the variation of 1mpatiens balsamina could be caused by the injection of low energy ions, and it was a useful way in flower industry. |
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| Keywords: | ions implantation Impatiens balsamina L random amplified polymorphic DNA |
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