| 竞争性PCR定量检测鸡传染性贫血病毒核酸 |
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| 引用本文： | 刘泽文,徐涤平,周平华,杨峻,段正赢,汪宏才,邵华斌,山口成夫.竞争性PCR定量检测鸡传染性贫血病毒核酸[J].中国预防兽医学报,2005,27(3):227-229. |
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| 作者姓名： | 刘泽文 徐涤平 周平华 杨峻 段正赢 汪宏才 邵华斌 山口成夫 |
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| 作者单位： | 1. 湖北省农业科学院,畜牧兽医研究所,湖北,武汉,430209
2. 日本农林水产省,家畜卫生试验场,日本,筑波 |
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| 基金项目： | 湖北省科技攻关重点项目(2001AA201B03) |
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| 摘 要： | 将删除33个碱基序列的CAVDNA克隆进质粒pSBII,提取质粒DNA,经过纯化、定量后作为竞争性模板,建立了一种定量检测鸡传染性贫血病毒核酸方法。该方法能够检测出的最低CAVDNA为103.5Molecules/μL,其精确度可达到100.5Molecules/μL。该方法与传统方法相比,具有节省时间、节约试剂和受其它因素干扰小等特点。
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| 关 键 词： | 竞争性PCR 定量检测 CAV |
| 文章编号： | 1008-0589(2005)03-0227-03 |
| 修稿时间： | 2004年4月8日 |
Quantification of chicken anemia virus by competitive polymerase chain reaction |
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| LIU Ze-wen,XU Di-ping,ZHOU Ping-hua,YANG Jun,DUAN Zheng-ying,WANG Hong-cai,SHAO Hua-bing,S.Yamaguchi.Quantification of chicken anemia virus by competitive polymerase chain reaction[J].Chinese Journal of Preventive Veterinary Medicine,2005,27(3):227-229. |
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| Authors: | LIU Ze-wen XU Di-ping ZHOU Ping-hua YANG Jun DUAN Zheng-ying WANG Hong-cai SHAO Hua-bing SYamaguchi |
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| Abstract: | Chicken anemia virus DNA in which 33 nucleotides were deleted was cloned into plasmid pSBII.The quantificational plasmid DNA was used as competitive template.A quantitive method was established to detect CAV DNA. The minimun DNA which can detect was 10~(3.5)molecules/uL and the accuracy was 10~(0. 5)molecules/uL.It has the specialty of saving time, regents and being influenced by few factors. |
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| Keywords: | competitive PCR quantification chicken anemia virus |
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