| 湘西黄牛粪源大肠埃希菌耐药性分析及多位点测序分型 |
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| 引用本文： | 汤起武,谭胜国,葛玲瑞,李运虎,田清武,余兴龙.湘西黄牛粪源大肠埃希菌耐药性分析及多位点测序分型[J].湖南农业大学学报(自然科学版),2022,48(1):87-94,119. |
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| 作者姓名： | 汤起武 谭胜国 葛玲瑞 李运虎 田清武 余兴龙 |
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| 作者单位： | 湖南生物机电职业技术学院动物科技学院,湖南 长沙 410127;湘西民族职业技术学院成教部,湖南 吉首 416007;湖南农业大学动物医学院,湖南 长沙 410128 |
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| 基金项目： | 湖南省自然科学基金科教联合项目(2018JJ5050)；湖南生物机电职业技术学院院级项目(20TD06) |
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| 摘 要： | 为了解湘西黄牛粪源大肠埃希菌的耐药情况,从湘西黄牛主产区花垣县和凤凰县6个不同规模的湘西黄牛养殖场采集黄牛肛门拭子样品,用麦康凯培养基和大肠埃希菌特异性引物分离、鉴定大肠埃希菌;采用K–B法对分离的大肠埃希菌进行5类共18种常用抗菌药物的敏感性试验;利用超高通量荧光定量PCR对16株多重耐药大肠埃希菌的7类共22种耐药...
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| 关 键 词： | 湘西黄牛 粪源 大肠埃希菌 耐药性 耐药基因 多位点测序分型 |
Drug resistance analysis and multi locus sequencing typing of fecal Escherichia coli from Xiangxi cattle |
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| TANG Qiwu,TAN Shengguo,GE Lingrui,LI Yunhu,TIAN Qingwu,YU Xinglong.Drug resistance analysis and multi locus sequencing typing of fecal Escherichia coli from Xiangxi cattle[J].Journal of Hunan Agricultural University,2022,48(1):87-94,119. |
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| Authors: | TANG Qiwu TAN Shengguo GE Lingrui LI Yunhu TIAN Qingwu YU Xinglong |
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| Abstract: | In order to understand the drug resistance of fecal Escherichia coli from Xiangxi cattle, anal swab samples of cattle were collected from six Xiangxi cattle farms of different scales in Huayuan County and Fenghuang County, the main production areas of Xiangxi cattle, and Escherichia coli was isolated and identified by maconkey medium and Escherichia coli specific primers; the sensitivity test of 18 kinds of common antibiotics in 5 categories was carried out on the isolated Escherichia coli by K-B method; 22 drug resistance genes of 7 classes of 16 strains of multidrug resistant Escherichia coli were detected by ultra-high throughput fluorescence quantitative PCR; multifocus sequence typing(MLST) of 16 isolates was carried out by 8 housekeeping genes of Escherichia coli, and ST cluster analysis and evolutionary tree analysis were carried out by goeBURST program and MEGA 7.0. The results showed that 170 strains of Escherichia coli were isolated and identified from 206 cattle anal swab samples; Escherichia coli isolated from cattle farms had high resistance to tetracycline, doxycycline, ampicillin, compound sulfamethoxazole and trimethoprim. The resistance rates of these five antibiotics in cattle farms with the highest resistance were 29.63%, 16.67%, 22.22%, 16.67% and 33.33%, respectively. There were 16 multi drug resistant bacteria and 8 drug resistance patterns. Six classes and eight kinds of drug resistance genes were detected, including acra-01(100.00%), fox5(81.25%), sul1(68.75%), sul2(75.00%), folA(100.00%), tetr-02(81.25%), inti-1(clinic)(68.75%) and tnpa-01(50.00%). A total of 11 ST types were obtained, and 9 new ST types were obtained. The 16 isolates were divided into 2 clonal groups and 7 haplotypes. All isolates were clustered into 2 groups, 2 branches and 7 branches, respectively. It could be concluded that the drug resistance of fecal Escherichia coli from cattle in Western Hunan was low. Multi drug resistant bacteria mainly appeared in large-scale farms and had a certain complexity. The monitoring of drug resistance of fecal Escherichia coli from large-scale cattle farms should be strengthened. |
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| Keywords: | Xiangxi cattle fecal source Escherichia coli drug resistance drug resistance gene MLST typing |
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