| 大口黑鲈组织蛋白酶B基因SNP筛选及其与生长性状关联分析 |
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| 引用本文： | 马冬梅,孙建国,费志平,樊佳佳,吴建开,白俊杰,周春龙,李胜杰.大口黑鲈组织蛋白酶B基因SNP筛选及其与生长性状关联分析[J].淡水渔业,2018(2):39-42. |
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| 作者姓名： | 马冬梅 孙建国 费志平 樊佳佳 吴建开 白俊杰 周春龙 李胜杰 |
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| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广州510380;2. 淡水水产健康养殖湖北省协同创新中心,武汉430070;3. 南京帅丰饲料有限公司,南京211306;4. 南京帅丰饲料有限公司,南京,211306;5. 湖州湖旺水产种业有限公司,浙江湖州,313000 |
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| 基金项目： | 南京市高端人才团队引进计划项目(2016)，浙江省农业(水产)新品种选育重大科技专项，渔港建设和渔业产业发展专项，广东省科技计划项目 |
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| 摘 要： | 在动物体内组织蛋白酶B(cathepsin B,CTSB)是以酶原形式存在于溶酶体中,能水解多种蛋白,是调控动物生长发育的重要候选功能基因。为开发与大口黑鲈(Micropterus salmoides)生长性状相关的单核苷酸多态性(single nucleotide polymorphism,SNP)标记,本研究对大口黑鲈EST-SNP库中CTSB基因上的SNP位点进行筛选。经PCR扩增和测序分析,从该基因中筛选到1个SNP位点(命名为C+598G),该位点属于错义突变位点,编码的甘氨酸突变成为精氨酸。采用Sna Pshot方法对C+598G位点在165尾大口黑鲈养殖样本中的基因型进行检测,并分析其在群体中的遗传多样性。结果显示,C+598G位点在养殖群体中存在CC、GC和GG3种基因型,其频率分别是40.6%、47.3%和12.1%,C和G等位基因频率分别是64.2%和35.8%;该位点在群体中的观测杂合度(Ho)和多态信息含量(PIC)分别为0.473和0.354,符合哈代-温伯格平衡定律。进一步分析不同基因型与大口黑鲈生长性状相关性,结果表明,3种基因型个体的体质量、全长、体高、尾柄长和尾柄高性状平均值从小到大依次为GG型、GC型和CC型,其中GG型个体在体质量上显著小于CC型个体(P<0.05)。本研究获得的C+598G标记与大口黑鲈生长性状显著相关,可作为大口黑鲈分子标记辅助育种研究中的生长标记。
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| 关 键 词： | 大口黑鲈(Micropterus salmoides) 组织蛋白酶B 单核苷酸多态性 生长性状 关联分析 |
SNP detection in cathepsin B gene and analysis of its correlations with growth traits in Micropterus salmoides |
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| MA Dong-mei,SUN Jian-guo,FEI Zhi-ping,FAN Jia-jia,WU Jian-kai,BAI Jun-jie,ZHOU Chun-long,LI Sheng-jie.SNP detection in cathepsin B gene and analysis of its correlations with growth traits in Micropterus salmoides[J].Freshwater Fisheries,2018(2):39-42. |
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| Authors: | MA Dong-mei SUN Jian-guo FEI Zhi-ping FAN Jia-jia WU Jian-kai BAI Jun-jie ZHOU Chun-long LI Sheng-jie |
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| Abstract: | Cathepsin B(CTSB)exits in the lysosome as the form of zymogen, which can hydrolyze different proteins and be a novel candidate functional gene that regulates growth and development in animals.SNP site in CTSB gene obtained from Micropterus salmoides EST-SNP library was screened to exploit SNP markers associated with growth traits.One SNP site(C+598G) was gained by PCR amplification and direct sequencing from CTSB gene coding region.This site was a missense mutation that changed a codon for glycine to a codon for arginine.The genotype of C+598G site in 165 M.salmoides indi-viduals was detected using SnaPshot method.The genetic diversity in the population was also analyzed.The results showed that C+598G site was constituted with CC, GC and GG genotype and their frequency in the population was 40.6%,47.3% and 12.1%, respectively.The frequency of C and G allele was 35.8% and 64.2% respectively.The observation heterozygosity(Ho)and polymorphic information content(PIC)of C+598G site was 0.473 and 0.354, respectively.This population was in the Hardy-Weinberg equilibrium for C+598G locus.Correlation analysis between different genotypes and M.salmoides growth traits showed that the order for the average body weight, total length, body depth, caudal pedun-cle length and caudal peduncle depth in different genotype was GG＜GC＜CC from small to large.The body weight of indi-viduals with GG genotype was significantly lighter than that of CC genotype(P＜0.05).The C+598G gained in this re-search was significantly related to growth traits, which can be useful in marker assisted breeding for M.salmoides. |
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