| 温度和pH值对河鲈消化酶活性的影响 |
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| 引用本文： | 范镇明,钱龙,王咏星.温度和pH值对河鲈消化酶活性的影响[J].淡水渔业,2008,38(2):36-39. |
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| 作者姓名： | 范镇明 钱龙 王咏星 |
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| 作者单位： | 1. 新疆兵团水产技术推广站,乌鲁木齐,830002
2. 新疆大学生命科学与技术学院,鸟鲁木齐,830046 |
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| 基金项目： | 新疆生产建设兵团科技攻关计划 |
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| 摘 要： | 研究了离体状态下河鲈(Perca fluviatilis)体内淀粉酶、蛋白酶和脂肪酶在不同温度和pH时的活性变化。结果显示:在河鲈肝胰脏、肠道、胃3部位,淀粉酶的最适温度均为30℃,最适pH值分别为5.0、7.0、8.0;脂肪酶的最适温度均为40℃,最适pH值分别为4.0、3.0、6.0;肝胰脏、肠道蛋白酶的最适温度为50℃,胃蛋白酶的最适温度为40℃。肝胰脏、肠和胃蛋白酶的最适pH值分别6.0、8.0、2.0。在各自最适温度下,肠道中脂肪酶比活力最高,其次是肝胰脏和胃;肝胰脏淀粉酶比活力最高,其次是肠道和胃;肠道中的蛋白酶比活力最高,其次是胃和肝胰脏。
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| 关 键 词： | 河鲈(Perca fluviatilis) 消化酶活性 温度 pH |
| 文章编号： | 1000-6907-（2008）01-0036-04 |
| 收稿时间： | 2007-11-03 |
| 修稿时间： | 2007年11月3日 |
Effects of Different Temperature and pH on Digestive Enzyme in Perca fluviatilis |
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| FAN Zhen-ming,QIAN Long,WANG Yong-xing.Effects of Different Temperature and pH on Digestive Enzyme in Perca fluviatilis[J].Freshwater Fisheries,2008,38(2):36-39. |
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| Authors: | FAN Zhen-ming QIAN Long WANG Yong-xing |
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| Abstract: | By changing temperature and pH,the amylase,protease and lipase of Perca fluviatilis were studied in vitro.The results shown that the optimum temperature of amylase was 30 ℃ for hepatopancreas,intestine and stomach,and their relevantly optimum pH was 5.0,7.0 and 8.0,respectively.The optimum temperature of Lipase from three digest organs was all 40 ℃,and their optimum pH were 4.0,3.0 and 6.0,respectively.The optimum temperature of Protease was 50 ℃ from in hepatopancreas and intestine,40 ℃ in stomach,while their optimum pH was 6.0,8.0 and 2.0,respectively.At each optimum temperature,the order of activity of lipase was intestine,hepatopancreas and stomach.Amylase′s activity order was hepatopancreas,intestine and stomach.Protease′s was intestine,stomach and hepatopancreas. |
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| Keywords: | pH |
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