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| 枯草芽孢杆菌Bs-916胞外抗菌蛋白质的生物功能分析 | |
| 引用本文： | 刘永锋,张杰,陈志谊,周明国,宋福平,刘邮洲,聂亚锋,罗楚平.枯草芽孢杆菌Bs-916胞外抗菌蛋白质的生物功能分析[J].江苏农业学报,2008,24(3). |
| 作者姓名： | 刘永锋 张杰 陈志谊 周明国 宋福平 刘邮洲 聂亚锋 罗楚平 |
| 作者单位： | 1. 江苏省农业科学院植物保护研究所,江苏,南京,210014 2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100094 3. 南京农业大学植物保护学院,江苏,南京,210095 |
| 基金项目： | 江苏省自然科学基金，国家重点实验室基金 |
| 摘 要： | 采用AKTA-epxlore层析系统,利用DEAE sepharose fast flow、Phenyl sepharose 6 F.F.和羟基磷石灰石柱层析后,分离纯化获得枯草芽孢杆菌Bs-916抗菌蛋白质Bacisubin.该蛋白质对水稻纹枯病菌(Rhizoctonia solani)、水稻稻瘟病菌(Magnaporthe grisease)、油菜菌核病菌(Sclerotinia sclerotiorum)、白菜黑斑病菌(Alternaria oleracea)、甘蓝黑斑病菌(A. Brassicae)、灰霉病菌(Botrytis cinerea)、辣椒炭疽病菌(Colletotrichum capsici (syd.) Butl.)和水稻恶苗病菌(Fusarium moniliforme Sheld)均有抑菌活性,尤其对Alternaria属的病原菌具有较高的毒力.枯草芽孢杆菌Bs-916胞外抗菌蛋白质的抑菌机制是造成病原菌菌丝营养吸收困难,致使菌丝顶端膨大,分支增加,胞壁加厚、断裂,从而抑制了病原菌的生长.研究发现抗菌蛋白具有凝集素活性和核糖核酸酶活性,无蛋白酶活性和蛋白酶抑制活性. |
| 关 键 词： | 抗菌蛋白质 抑菌谱 生物功能 枯草芽孢杆菌Bs-916 |
Analysis of Bioactivities of Antifungal Protein(Bacisubin) Isolated from Bacillus subtilis Strain Bs-916 |
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| LIU Yong-feng,ZHANG Jie,CHEN Zhi-yi,ZHOU Ming-guo,SONG Fu-ping,LIU You-zhou,NIE Ya-feng,LUO Chu-ping.Analysis of Bioactivities of Antifungal Protein(Bacisubin) Isolated from Bacillus subtilis Strain Bs-916[J].Jiangsu Journal of Agricultural Sciences,2008,24(3). | |
| Authors: | LIU Yong-feng ZHANG Jie CHEN Zhi-yi ZHOU Ming-guo SONG Fu-ping LIU You-zhou NIE Ya-feng LUO Chu-ping |
| Abstract: | This paper reported the antifungal activity of antifungal protein(bacisubin) toward Rhizoctonia solani,Magnaporthe grisease,Sclerotinia sclerotiorum,Alternaria oleracea,A.brassicae,Botrytis cinerea,Colletotrichum capsici(syd.) Butl and Fusarium moniliforme Sheld.The results exhibited that the antifungal protein had a broad-spectrum of antifungal activities and high virulence.Especially,the antifungal protein exhibited a stronger antifungal to pathogenic fungi,such as generic Alternaria.The mechanism to antifungal activity of bacisubin caused mycelia alimentation obstacle.Mycelia apex appeared distortion,tumescence,rupture and cell wall thickened.However,bacisubin did not show hemagglutinating activity,protease activity,protease inhibitory activity,but exhibited a certain ribonuclease activity. |
| Keywords: | antifungal protein antifungal activity bioactivity Bacillus subtilis strain Bs-916 |
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