A two-step species-specific 16S rRNA PCR assay for the detection of Taylorella equigenitalis in horses |
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| Authors: | Thomas C Buckley B Cherie Millar Claire L Egan Paula Gibson Hazel Cosgrove Siobhan Stanbridge Motoo Matsuda John E Moore |
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| Institution: | Irish Equine Centre, Johnstown, Naas, Co Kildare, Republic of Ireland. iec@equine-centre.ie. |
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| Abstract: | : A two-step PCR assay was developed for the molecular detection of Taylorella equigenitalis, a Gram-negative genital bacterial pathogen in horses. Two specific oligonucleotide primers (TE16SrRNABCHf 25mer] and TE16SrRNABCHr 29mer]) were designed from multiple alignments of the 16S rRNA gene loci of several closely related taxa, including T. asinigenitalis. Subsequent enhanced surveillance of 250 Thoroughbred animals failed to detect the presence of this organism directly from clinical swabs taken from the genital tract of mares and stallions. Such a molecular approach offers a sensitive and specific alternative to conventional culture techniques, and has the potential to lead to improved diagnosis and subsequent management of horses involved in breeding programmes. |
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| Keywords: | Horse Taylorella equigenitalis 16S rRNA Contagious equine metritis Polymerase chain reaction |
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