| 转基因玉米种子萌发期抗旱性鉴定 |
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| 引用本文： | 冷益丰,张彪,赵久然,杨俊品,刘亚,康继伟,陈洁,唐海涛,谭君,何文铸.转基因玉米种子萌发期抗旱性鉴定[J].干旱地区农业研究,2013,31(1):177-182. |
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| 作者姓名： | 冷益丰 张彪 赵久然 杨俊品 刘亚 康继伟 陈洁 唐海涛 谭君 何文铸 |
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| 作者单位： | 四川省农业科学院作物研究所;北京市农林科学院 |
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| 基金项目： | 转基因生物新品种培育重大专项(2009ZX08003-007B);国家玉米支撑计划(2011BAD35B01);国家玉米863计划(2011AA10A103-2);四川省农科院青年基金(2011QNJJ-008) |
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| 摘 要： | 采用聚乙二醇(PEG)高渗溶液,对13个转基因玉米自交系进行了种子萌发期模拟水分胁迫发芽试验,研究了渗透胁迫模拟干旱对抗旱转基因玉米种子萌发状况的影响。结果表明:干旱胁迫对玉米发芽势、发芽率、根数、胚根长、胚芽长、贮藏物质转化率等均有不同程度的影响,不同转基因玉米自交系在抗旱性上存在明显差异。同时,使用隶属函数法对参试转基因玉米自交系的种子萌发抗旱性进行了分析评价,其中SD10为萌发期抗旱性较强的自交系;CBF-3-4、SD13、SD05、CBF-1-4、CBF-6-5、SD-P3、SD-P5为萌发期抗旱性中等的自交系;SD06、CBF-3-1、SD07、SD-P1、SD-P4为萌发期抗旱性较弱的自交系。种子萌发抗旱指数与隶属函数对玉米自交系抗旱性分析结果比较一致。
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| 关 键 词： | 转基因玉米 种子萌发 渗透胁迫 隶属函数 |
Identification of drought resistance of transgenic maize during
seed germination stage |
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| LENG Yifeng,ZHANG Biao,ZHAO Jiuran,YANG Junpin,LIU Y,KANG Jiwei,CHEN Jie,TANG Haitao,TAN Jun,HE Wenzhu.Identification of drought resistance of transgenic maize during
seed germination stage[J].Agricultural Research in the Arid Areas,2013,31(1):177-182. |
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| Authors: | LENG Yifeng ZHANG Biao ZHAO Jiuran YANG Junpin LIU Y KANG Jiwei CHEN Jie TANG Haitao TAN Jun HE Wenzhu |
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| Institution: | 1(1.The Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610066,China; 2.Beijing Academy of Agricultural & Forestry Science,Beijing 100097,China) |
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| Abstract: | The experiment was carried out with 13 transgenic inbred lines of maiz
e under the polyethylene glycol (PEG) simulated water stress to study the impact
of osmotic stress on seed germination. The results showed that the germination
potential, germination rate, root number, radicle length, plumule length and tra
nsforming rate of storage substance in maize were affected by drought stress to
different extend, and there existed significant differences in drought resistanc
e among different inbred lines. The quantitative evaluation by using subordinate
function method indicated that SD10 was the inbred line with high drought-resis
tance; CBF-3-4, SD13, SD05, CBF-1-4, CBF-6-5, SD-P3 and SD-P5 were the inbred li
nes with mid drought-resistance; and SD06, CBF-3-1, SD07, SD-P1 and SD-P4 were the inbred lines with low drought-resistance. The analysis results obtained by grain drought resistant index (GDRI) were similar to those by subordinate function method. |
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| Keywords: | transgenic maize seed germination osmotic stress subordinate function |
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