| 二色胡枝子遗传转化中抗生素浓度优化研究 |
| |
| 引用本文： | 李明,李伟,杨晓红,黄天伟,陈晓阳.二色胡枝子遗传转化中抗生素浓度优化研究[J].广东农业科学,2009(5):44-47. |
| |
| 作者姓名： | 李明 李伟 杨晓红 黄天伟 陈晓阳 |
| |
| 作者单位： | 李明,李伟,黄天伟(北京林业大学林木花卉遗传育种教育部重点实验室,北京,100083);杨晓红(北京农学院园林学院,北京,102206);陈晓阳(华南农业大学林学院,广东广州,510642) |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)项目 |
| |
| 摘 要： | 探讨了卡那霉素、G418和头孢霉素3种抗生素对二色胡枝子不同培养阶段外植体生长、分化和生根的影响及对农杆菌的抑菌效果.确立了由农杆菌介导的二色胡枝子遗传转化研究中抗生素转化体的筛选浓度和头孢霉素对菌株的抑菌浓度.结果表明:在子叶节转化筛选阶段,卡那霉素和G418的适宜浓度分别为20 mg/L和7.5 mg/L;在抗性芽增殖培养阶段.卡那霉素和G418浓度分别为150 mg/L和15.0 mg/L;在生根阶段,卡那霉素和G418浓度分别为20 mg/L和5.0mg/L.而头孢霉素在各阶段适宜浓度为0～300 ms/L;头孢霉素对农杆菌LBA4404和C58抑制浓度为200～300 mg/L时效果理想.
|
| 关 键 词： | 二色胡枝子 抗生素浓度优化 遗传转化 |
Optimization of antibiotic concentration during genetic transformation of Lespedeza bicolor |
| |
| LI Ming,LI Wei,YANG Xiao-hong,HUANG Tian-wei,CHEN Xiao-yang.Optimization of antibiotic concentration during genetic transformation of Lespedeza bicolor[J].Guangdong Agricultural Sciences,2009(5):44-47. |
| |
| Authors: | LI Ming LI Wei YANG Xiao-hong HUANG Tian-wei CHEN Xiao-yang |
| |
| Institution: | LI Ming1,LI Wei1,YANG Xiao-hong3,HUANG Tian-wei1,CHEN Xiao-yang2(1.Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083,China,2.Forestry College,South China Agricultural University,Guangzhou 510642,3.Landscape College,Beijing University of Agriculture,Beijing 102206,China) |
| |
| Abstract: | In this study,we carried out a research about the effects of three antibiotics of kanamycin,G418 and cephalothin on growing,differentiation and rooting of tissue culture seedling of Lespedeza bicolor,and we also determined inhibitive effect of cephalothin on the growth of Agrobacterium.After data analysing,we established the optimal antibiotics concentration at different stages for transgenic explant selection during genetic transformation of Lespedeza bicolor conducted by agrobacterium.The results were as ... |
| |
| Keywords: | Lespedeza bicolor antibiotic concentration optimization genetic transformation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
| 点击此处可从《广东农业科学》下载免费的PDF全文 |
|
