| 化学合成寡糖诱导烟草抗黑胫病的初步研究 |
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| 引用本文： | 朱振元,张勇民,徐同.化学合成寡糖诱导烟草抗黑胫病的初步研究[J].植物病理学报,2004,34(3):231-236. |
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| 作者姓名： | 朱振元 张勇民 徐同 |
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| 作者单位： | 1 浙江大学农业与生物技术学院植保系, 杭州 310029;2 Ecole Normale Supérieure, Département de Chimie, Associé au CNRS, 24, Rue Lhomond, 75231 Paris Cedex 05, France;3 贵州大学生物技术与环境资源学院生物系, 贵阳 550025 |
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| 摘 要： | 抑菌测定和盆栽试验表明:化学合成的Lewisx和Lewisa寡糖对烟草黑胫病病原菌菌丝生长无明显影响;Lewisx五糖和七糖处理烟草,可使烟草植株产生对黑胫病菌(Phytophthora nicotianae)的抗性,在处理浓度0~10μg/m L内,随着浓度增高诱导效果增强,当浓度达10 μg/m L时,诱导防病效果达90%以上。Lewisx七糖处理,可导致烟草叶片过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸裂解酶(PAL)等防卫酶活性不同程度地提高,PPO、POD及PAL分别在处理后第4d、第6d和第8d达到最大值,比对照分别增加了70.7%、150.0%和142.8%。
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| 关 键 词： | 寡糖 烟草黑胫病 诱导抗病性 防卫酶 |
| 文章编号： | 0412-0914(2004)03-0231-06 |
| 修稿时间： | 2003年6月27日 |
Induced resistance of tobacco to Phytophthora nicotianae by synthetic oligosaccharides |
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| ZHU Zhen-yuan ,ZHANG Yong-min,XU Tong.Induced resistance of tobacco to Phytophthora nicotianae by synthetic oligosaccharides[J].Acta Phytopathologica Sinica,2004,34(3):231-236. |
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| Authors: | ZHU Zhen-yuan ZHANG Yong-min XU Tong |
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| Institution: | 1 Department of Plant Protection, Zhejiang University, Hangzhou 310029, China;2 Ecole Normale Supérieure, Département de Chimie, Associé au CNRS, 24, Rue Lhomond, 75231 Paris Cedex 05, France;3 Laboratory of Fungus Resources, Guizhou University, Guiyang 550025, China |
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| Abstract: | The synthetic oligosaccharides induced tobacco resistance against P. nicotianae and did not inhibit the mycelial growth of the pathogen in vitro. The induced resistance of tobacco plants treated with Lewisx quinquesaccharide or heptasaccharide was related to concentrations used in the range 0-10 μg/mL, treated plants showed the maxium induction efficiency (97.9%) at 10 μg/mL. The activities of three defense enzymes, polyphenoloxidase, peroxidase and phenylalanine ammoniayase in tobacco plants treated with Lewisx heptasaccharide at 10 μg/mL increased by 70.7%, 150.0% and 142.8%, respectively. The mechanism of the induced resistance is discussed. |
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| Keywords: | oligosaccharides Tobacco black shank induced resistance defense enzymes |
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