| 3,5-二硝基水杨酸比色法快速测定烟草水溶性总糖、还原糖及淀粉的探讨 |
| |
| 引用本文： | 尹建雄,卢 红,谢 强,丁金玲,李尼杭.3,5-二硝基水杨酸比色法快速测定烟草水溶性总糖、还原糖及淀粉的探讨[J].云南农业大学学报,2007,22(6):829-833. |
| |
| 作者姓名： | 尹建雄 卢 红 谢 强 丁金玲 李尼杭 |
| |
| 作者单位： | [1]石林县天合烟叶复烤有限责任公司,云南石林652200 [2]云南农业大学烟草学院,云南昆明650201 |
| |
| 摘 要： | 通过还原糖对3,5-二硝基水杨酸的还原产物的吸收光谱分析，结果表明，该还原产物在500 nm波长下有最大吸收峰，但最佳的工作波长确定为540 nm。同时找出了显色反应时间及显色剂的最佳用量。并对烟草中水溶性总糖的提取方法进行了优化，建立了批量连续测定烟样中还原糖、水溶性总糖（进行酸水解）、淀粉的快速方法。同时对多个烟样进行了测定，与伯川法测定结果比较，其相对误差在5%以内。
|
| 关 键 词： | 烟草 比色法 5-二硝基水杨酸 |
| 文章编号： | 1004-390X（2007）06-0829-05 |
| 收稿时间： | 2006-9-14 |
| 修稿时间： | 2006-09-14 |
A Study on Rapid Colorimetric Determination of Water Soluble Total Sugar, Reducing Sugar and Starch in Tobacco with 3,5-dinitrosalicylic Acid |
| |
| YIN Jian-xiong,LU Hong,XIE Qiang,DING Jin-ling,LI Ni-hang.A Study on Rapid Colorimetric Determination of Water Soluble Total Sugar, Reducing Sugar and Starch in Tobacco with 3,5-dinitrosalicylic Acid[J].Journal of Yunnan Agricultural University,2007,22(6):829-833. |
| |
| Authors: | YIN Jian-xiong LU Hong XIE Qiang DING Jin-ling LI Ni-hang |
| |
| Institution: | 1. TIANGHE Tobacco Threshing LID, CO. Stone Forest 652200, China ; 2. Faculty of Tobacco Science, Yunnan Agricultural University, Kunming 650201, China |
| |
| Abstract: | The absorption spectrum of 3,5-dinitrosalicylic acid reducing product with reducing sugar has been studied.The result shows a maximum absorption at 500 nm,but the best work wavelength was determined by 540 nm.The best time of color reaction and best suitable amount of the color reagent was given.The extraction methods of water soluble total sugar was optimized,and a rapid continuous batch determination of reducing sugar,water soluble total sugar and starch was established.When some tobacco samples were determined.The relative errors of the results obtained by this method related to those obtained by the Bertrand's method were under of 5%. |
| |
| Keywords: | tobacco colorimetry 3 5-dinitrosalicylic acid |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《云南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《云南农业大学学报》下载免费的PDF全文 |
|
