| 反刍月形单胞菌乙酸生成缺陷株的构建及相关酶活性分析(英文) |
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| 引用本文： | 苑学,逄晓阳,刘国文,王哲.反刍月形单胞菌乙酸生成缺陷株的构建及相关酶活性分析(英文)[J].Agricultural Science&Technology,2011(8). |
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| 作者姓名： | 苑学 逄晓阳 刘国文 王哲 |
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| 作者单位： | 吉林大学农学部畜牧兽医学院;内蒙古民族大学动物科学技术学院;国家农业深加工产品质量监督检验中心; |
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| 基金项目： | Supported by National Natural Science Fund Item(30230260,30600441)~~ |
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| 摘 要： | 目的]构建反刍月形单胞菌乙酸生成的缺陷株并分析其发酵特性。方法]应用转座子标签法,通过转座子供体菌E.coliS17-1/pZJ25∷Tn5对受体菌反刍月形单胞菌进行转座子诱变,采用含卡那霉素和氟乙酸纳的选择性培养基筛选接合子。结果]共筛选出稳定的对卡那霉素和氟乙酸具有抗性的转座工程菌7株。对反刍月形单胞菌的突变株进行16SrRNA鉴定和Tn5的PCR鉴定,及乙酸激酶(AK)和磷酸乙酰转移酶(PTA)酶比活力分析,确定突变株属于pta基因缺失型氟乙酸抗性菌株。结论]为进一步研究反刍兽瘤胃微生物乙酸的细胞代谢网络和调控奠定基础。
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| 关 键 词： | 反刍月形单胞菌 转座子 磷酸乙酰转移酶 乙酸激酶 |
Construction of Deficient Strain by Selenomonas ruminantium Mutant with the Acetic Acid and Analysis on Its Related Enzyme Activity |
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| YUAN Xue,PANG Xiao-yang,LIU Guo-wen,WANG Zhe .College of Animal Sciences , Veterinary Medicine,Jilin University,Changchun ,.College of Animal Science , Technology,Inner Mongolia University for Nationalities,Tongliao ,.China National Quality Supervision Test Center for Processed Agricultural Products,Changchun.Construction of Deficient Strain by Selenomonas ruminantium Mutant with the Acetic Acid and Analysis on Its Related Enzyme Activity[J].农业科学与技术(英文版),2011(8). |
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| Authors: | YUAN Xue PANG Xiao-yang LIU Guo-wen WANG Zhe College of Animal Sciences Veterinary Medicine Jilin University Changchun College of Animal Science Technology Inner Mongolia University for Nationalities Tongliao China National Quality Supervision Test Center for Processed Agricultural Products Changchun |
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| Institution: | YUAN Xue1,2,PANG Xiao-yang1,3,LIU Guo-wen1,WANG Zhe1 1.College of Animal Sciences and Veterinary Medicine,Jilin University,Changchun 130062,2.College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028000,3.China National Quality Supervision Test Center for Processed Agricultural Products,Changchun 130022 |
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| Abstract: | Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.Method]Based on the transposon tagging method,Selenomonas ruminantium(recipient strain)was carried out the transposon mutagenesis via the transposon donor strain E.coli S17-1/pZJ25∷Tn5.The zygote was screened by using the selective medium which included kanamycin and sodium fluoroacetate.Result]Seven transposon engineered strains which had the stable resistance to kanamycin and fluoroethanoic acid were screened.Selenomonas ruminantium mutant was carried out 16S rRNA and Tn5 PCR identification.Moreover,the specific activities of AK and PTA were analyzed.The mutant belonged to fluoroethanoic acid resistance strain with pta gene deficiency.Conclusion]The research laid the foundation for further studying the cellular metabolic network and regulation of acetic acid in rumen microorganism of ruminant animal. |
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| Keywords: | Selenomonas ruminantium Transposon PTA AK |
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