| 猪口蹄疫O型合成肽疫苗抗原多肽的串联表达及活性鉴定 |
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| 引用本文： | 卢清侠,郭军庆,郝慧芳,杨苏珍,邢广旭,杨继飞,王世红,张改平.猪口蹄疫O型合成肽疫苗抗原多肽的串联表达及活性鉴定[J].河南农业科学,2011,40(10):126-130. |
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| 作者姓名： | 卢清侠 郭军庆 郝慧芳 杨苏珍 邢广旭 杨继飞 王世红 张改平 |
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| 作者单位： | 河南省农业科学院农业部动物免疫学重点实验室/河南省动物免疫学重点实验室,河南郑州,450002 |
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| 基金项目： | 国家“863”计划(2007AA100606); 中国博士后科学基金面上资助项目(20110491000) |
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| 摘 要： | 为获得含合成肽疫苗抗原多肽的重组表达蛋白,根据猪口蹄疫O型合成肽疫苗中抗原多肽的氨基酸序列,使用大肠杆菌偏好性密码子人工合成该抗原多肽的双拷贝基因序列,并克隆至表达载体pET32a中,构建原核表达质粒pET32a - P2.将重组质粒转化BL21 (DE3),经IPTG诱导表达,收集诱导的菌液进行SDS - PAGE电...
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| 关 键 词： | 猪口蹄疫 合成肽疫苗 抗原多肽 串联表达 蛋白纯化 活性鉴定 |
Tandem Expression and Activity Analysis of Antigen Peptide of Swine Foot and Mouth Disease(Type O) Synthetic Peptide Vaccine |
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| LU Qing-xia,GUO Jun-qing,HAO Hui-fang,YANG Su-zhen,XING Guang-xu,YANG Ji-fei,WANG Shi-hong,ZHANG Gai-ping.Tandem Expression and Activity Analysis of Antigen Peptide of Swine Foot and Mouth Disease(Type O) Synthetic Peptide Vaccine[J].Journal of Henan Agricultural Sciences,2011,40(10):126-130. |
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| Authors: | LU Qing-xia GUO Jun-qing HAO Hui-fang YANG Su-zhen XING Guang-xu YANG Ji-fei WANG Shi-hong ZHANG Gai-ping |
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| Institution: | LU Qing-xia,GUO Jun-qing,HAO Hui-fang,YANG Su-zhen,XING Guang-xu,YANG Ji-fei,WANG Shi-hong,ZHANG Gai-ping (Key Laboratory of Animal Immunology of the Ministry of Agriculture/Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China) |
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| Abstract: | In order to get the recombination protein which contained antigen peptide of synthetic peptide vaccine,two-copy gene was synthesized using E.coli optimal codons according to the amino acid sequence of antigen peptide.The recombinant expression vector pET32a-P2 was constructed by cloning two-copy gene of antigen peptide into the prokaryotic expression plasmid pET32a.The BL21(DE3) was transformed with pET32a-P2,and the protein corresponding to P2 gene was expressed after induction with IPTG.SDS-PAGE and Weste... |
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| Keywords: | Swine foot and mouth disease Synthetic peptide vaccine Antigen peptide Tandem expression Protein purification Activity analysis |
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