| 蓝藻伪空胞的测定及其前处理方法(英文) |
| |
| 引用本文： | 杨波,储昭升,潘纲.蓝藻伪空胞的测定及其前处理方法(英文)[J].Agricultural Science&Technology,2011(8). |
| |
| 作者姓名： | 杨波 储昭升 潘纲 |
| |
| 作者单位： | 中国科学院生态环境研究中心;海南大学三亚学院;国家环境保护湖泊污染控制重点实验室中国环境科学研究院湖泊生态环境创新基地; |
| |
| 基金项目： | Supported by Chinese National Fundamental Research Program(973Program)(2008CB418105)~~ |
| |
| 摘 要： | 目的]探讨蓝藻伪空胞的测定及其前处理方法。方法]改进了测定伪空胞的毛细管压力法,对改进后的装置进行了检测限和精密度测试,并研究了2种浓缩方法和2种保存方法对3种蓝藻细胞数量和伪空胞含量的影响。结果]改进后的蓝藻伪空胞测定装置其检测限达到0.0018μl/ml藻液,同一样品测定结果相对标准偏差小于1%。采用过滤和离心2种浓缩方法时,分散单细胞的微囊藻细胞损失都大于50%,其中小群体的微囊藻采用离心的方法细胞损失较小,而丝状的颤藻采用过滤的方法损失较小;这2种方法对伪空胞含量的影响均可忽略。3种蓝藻在直接冷藏和添加鲁哥试剂保存7d后,其细胞浓度和单位细胞的伪空胞含量均未发生明显改变,其中对于伪空胞,直接冷藏的损失更小;对于自然水体样品,宜添加鲁哥试剂进行保存。结论]为研究蓝藻浮力调节机制及其暴发机理提供了理论依据。
|
| 关 键 词： | 蓝藻 浓缩 保存 伪空胞 |
Measurement of Gas Vesicle in Cyanobacteria and Its Pretreatment Methods |
| |
| YANG Bo,CHU Zhao-sheng,PAN Gang.Research Center for Eco-environmental Sciences,Chinese Academy of Sciences,Beijing ,.Hainan University Sanya College,Sanya ,.State Environmental Protection Key Laboratory for Lake Pollution Control,Research Center of Lake Eco-environments,Chinese Research Academy of Environmental Sciences,Beijing.Measurement of Gas Vesicle in Cyanobacteria and Its Pretreatment Methods[J].农业科学与技术(英文版),2011(8). |
| |
| Authors: | YANG Bo CHU Zhao-sheng PAN GangResearch Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing Hainan University Sanya College Sanya State Environmental Protection Key Laboratory for Lake Pollution Control Research Center of Lake Eco-environments Chinese Research Academy of Environmental Sciences Beijing |
| |
| Institution: | YANG Bo1,2,CHU Zhao-sheng3,PAN Gang11.Research Center for Eco-environmental Sciences,Chinese Academy of Sciences,Beijing 100085,2.Hainan University Sanya College,Sanya 572022,3.State Environmental Protection Key Laboratory for Lake Pollution Control,Research Center of Lake Eco-environments,Chinese Research Academy of Environmental Sciences,Beijing 100012 |
| |
| Abstract: | Objective]The aim was to measure gas vesicle in cyanobacteria and discuss its pretreatment methods.Method]The capillary pressure method to determine gas vesicle in cyanobacteria was modified firstly,and then the lower detection limit and precision of modified apparatus were tested,finally the effects of two concentration methods and preservation methods on cell quantity and gas vesicle content of three cyanobacterias were studied.Result]The lower detection limit and precision of modified apparatus to mea... |
| |
| Keywords: | Cyanobacteria Concentration Preservation Gas vesicle |
| 本文献已被 CNKI 等数据库收录！ |
|
