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| 条斑紫菜半胱氨酸合成酶基因表达对重金属铅胁迫的响应 | |
| 引用本文： | 周向红,李信书,阎斌伦,易乐飞,王萍.条斑紫菜半胱氨酸合成酶基因表达对重金属铅胁迫的响应[J].河南农业科学,2011,40(10):111-114. |
| 作者姓名： | 周向红 李信书 阎斌伦 易乐飞 王萍 |
| 作者单位： | 1. 淮海工学院江苏省海洋生物技术重点实验室,江苏连云港222005;淮海工学院海洋学院,江苏连云港222005 2. 淮海工学院海洋学院,江苏连云港,222005 3. 淮海工学院江苏省海洋生物技术重点实验室,江苏连云港,222005 |
| 基金项目： | 江苏省海洋生物技术重点实验室开放课题(2008HS004); 淮海工学院自然科学研究项目(2010150023) |
| 摘 要： | 为了研究条斑紫菜的重金属解毒机制,分别采用Pb2+1、4、7、10 mg/L对条斑紫菜叶状体进行短期铅胁迫,同时,用10 mg/L的pb2+对叶状体进行持续铅胁迫,胁迫处理后提取总RNA并采用实时荧光定量PCR技术检测条斑紫菜半胱氨酸合成酶基因(PyCSase -B)的表达变化.结果显示,铅胁迫能迅速且显著诱导PyCS... |
| 关 键 词： | 条斑紫菜 丰胱氨酸合成酶 基因表达 铅胁迫 |
Response of Cysteine Synthase Gene Expression to Lead Stress in Porphyra yezoensis Ueda |
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| ZHOU Xiang-hong,LI Xin-shu,YAN Bin-lun,YI Le-fei,WANG Ping.Response of Cysteine Synthase Gene Expression to Lead Stress in Porphyra yezoensis Ueda[J].Journal of Henan Agricultural Sciences,2011,40(10):111-114. | |
| Authors: | ZHOU Xiang-hong LI Xin-shu YAN Bin-lun YI Le-fei WANG Ping |
| Institution: | ZHOU Xiang-hong1,2,LI Xin-shu2,YAN Bin-lun1,YI Le-fei1,WANG Ping2(1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China,2.School of Marine Science & Technology,China) |
| Abstract: | To investigate the mechanism of heavy metal detoxification in intertidal red alga Porphyra yezoensis Udea,the expression of cysteine synthase gene(PyCSase-B) in P.yezoensis thalli responding to lead stress was examined.Thalli were subjected to seawater treatment with different concentration of lead(1,4,7,10 mg/L Pb2+)for 2 h,and then subjected to seawater treatment with 10 mg/L Pb2+ for 12 h.Then total RNA was extracted from thalli,and the relative mRNA levels of PyCSase-B were examined using real-time fluo... |
| Keywords: | Porphyra yezoensis Ueda Cysteine synthase Gene expression Lead stress |
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