| 检测H9亚型AIV的压电免疫传感器研究(英文) |
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| 引用本文： | 詹爱军,胡云发,王新卫,刘靖清,卞红春,陈枝楠.检测H9亚型AIV的压电免疫传感器研究(英文)[J].Agricultural Science&Technology,2011(10). |
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| 作者姓名： | 詹爱军 胡云发 王新卫 刘靖清 卞红春 陈枝楠 |
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| 作者单位： | 深圳市检验检疫科学研究院;河南农业大学牧医工程学院;盐城市农业委员会; |
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| 基金项目： | Supported by the Supporting Program of theEleventh Five-year Planfor Sci&Tech Research of China(2006BAK20A29); Strategical Project for Science and Technology of Guangdong Province(2004A2090102)~~ |
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| 摘 要： | 目的]研制检测H9亚型禽流感病毒的压电免疫传感器。方法]用巯基丙酸在镀银电极石英晶体自组装巯基丙酸单分子膜再通过N-乙基-N'-(3-二甲氨基)丙基碳化二亚胺盐酸(EDC)和N-羟基琥珀酰亚胺(NHS)偶联抗H9亚型禽流感病毒的特异性单抗构建传感器芯片,建立可以检测H9N2亚型禽流感病毒的免疫传感器。结果]该方法具有较好的特异性,不与H5亚型流感病毒和新城疫病毒(NDV)反应,检测灵敏度达到20～100个EID50。结论]该结果为检测禽流感病毒免疫传感器的进一步深入研究奠定了基础,这为其他相关病毒的监测提供了一种新途径。
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| 关 键 词： | 压电免疫传感器 生物自组装法 H9亚型禽流感病毒 |
Study on Piezoelectric Immunosensor for the Detection of H9-subtype Avian Influenza Virus |
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| ZHAN Ai-jun,HU Yun-fa,WANG Xin-wei,LIU Jing-qing,BIAN Hong-chun,CHEN Zhi-nan .Shenzhen Academy of Inspection , Quarantine,Shenzhen ,.College of Animal Husb,ry , Veterinary,Henan Agricultural University,Zhengzhou ,.Yancheng Agriculture Committee,Yancheng.Study on Piezoelectric Immunosensor for the Detection of H9-subtype Avian Influenza Virus[J].农业科学与技术(英文版),2011(10). |
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| Authors: | ZHAN Ai-jun HU Yun-fa WANG Xin-wei LIU Jing-qing BIAN Hong-chun CHEN Zhi-nan Shenzhen Academy of Inspection Quarantine Shenzhen College of Animal Husb ry Veterinary Henan Agricultural University Zhengzhou Yancheng Agriculture Committee Yancheng |
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| Institution: | ZHAN Ai-jun~1,HU Yun-fa~1,WANG Xin-wei~2,LIU Jing-qing~3,BIAN Hong-chun~3,CHEN Zhi-nan~1* 1.Shenzhen Academy of Inspection and Quarantine,Shenzhen 518010,2.College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,3.Yancheng Agriculture Committee,Yancheng 224002 |
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| Abstract: | Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus(AIV).Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid(MPA) to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N'-(3-dimethyl aminopropyl)carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS).The immunosensor to detect H9 subtype AIV was established.Result] The results showed... |
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| Keywords: | Piezoelectric immunosensor Biological self-assembly method H9 subtype avian influenza virus |
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