| 大鲵精液离体保存的初步研究 |
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| 引用本文： | 赵虎,张红星,王启军,陈玖安.大鲵精液离体保存的初步研究[J].水生态学杂志,2012,33(1):149-152. |
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| 作者姓名： | 赵虎 张红星 王启军 陈玖安 |
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| 作者单位： | 1. 陕西省动物研究所,陕西西安,710032
2. 宁陕龙泉大鲵驯养繁育场,陕西宁陕,711600 |
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| 基金项目： | 陕西省科学院基础应用研究项目（2009K-14） |
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| 摘 要： | 针对大鲵(Andrias davidianus)的性腺发育不同步，为提高受精率，对其精液特性和保存条件进行初步研究。人工辅助采集精液，对精液量、酸碱度、密度、活力等基本特性进行常规测定。分别对保护液、光照、温度等保存条件进行分组研究试验。结果表明：雄性亲鲵的精液量最大可达12 ml/kg，pH值6.7～7.0微酸状态，密度215～1075万/ml，精子活力为0.7～0.9。离体保存条件下，室温暗环境（20LUX）较光环境（400LUX）可延长存活时间0.5h，暗环境低温（0～4℃）较室温（21～22℃）可延长存活时间3d，低温暗环境原精液中添加保护液比对照组精子存活时间延长6～12h。
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| 关 键 词： | 大鲵(Andrias davidianus) 精液 离体保存 |
| 收稿时间： | 2010/10/12 0:00:00 |
| 修稿时间： | 12/6/2010 5:40:01 PM |
Preliminary Study on Conservation of Andrias davidanus Semen in Vitro |
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| zhao hu,zhang hong xing,wang qi jun and chen jiu an.Preliminary Study on Conservation of Andrias davidanus Semen in Vitro[J].Journal of Hydroecology,2012,33(1):149-152. |
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| Authors: | zhao hu zhang hong xing wang qi jun and chen jiu an |
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| Institution: | Shaanxi Institute of Zoology |
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| Abstract: | Gonadal development of Andrias davidanus not synchronized,semen characteristics and preservation conditions of A.davidanus were studied targeting the increase of fertility rate.Semina were collected by artificial assistance approach,and a regular assay was conducted on the basic features such as semen volume,pH,density,vitality,etc.Preservation conditions,namely,protection medium,illumination and temperature,were also studied.The results showed that semen volume of pro-male A.davidanus was up to 12 mL/kg,pH 6.7-7.0 of subacid condition;and that the semen density was(2.15-10.75)million/mL,sperm motility 0.7-0.9.In vitro conservation conditions,dark environment at ambient illuminance(20 lx).The result also indicated that the survival time of the semina was 0.5 h longer than those conserved in the luminous environment(400 lx);low temperature(0-4℃) could extend the survival time of the semina 3d longer than those at room temperature(21-22℃) in dark environment;in the cold dark environment,semina with protection medium added in could extend the survival time 6-12 h longer than the control group. |
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| Keywords: | Andrias davidanus semen conservation in vitro |
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