| 云南甘蔗花叶病病原检测及一个分离物的分子鉴定 |
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| 引用本文： | 李文凤,董家红,丁铭,方琦,黄应昆,苏晓霞,李婷婷,罗廷青,张仲凯.云南甘蔗花叶病病原检测及一个分离物的分子鉴定[J].植物病理学报,2007,37(3):242-247. |
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| 作者姓名： | 李文凤 董家红 丁铭 方琦 黄应昆 苏晓霞 李婷婷 罗廷青 张仲凯 |
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| 作者单位： | 1 云南省农业科学院甘蔗研究所, 开远 661600;2 云南省农业生物技术重点实验室, 昆明 650223 |
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| 基金项目： | 云南省重点实验室基金;云南省科技攻关及高新技术发展计划项目 |
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| 摘 要： | 调查表明甘蔗花叶病在云南发生普遍。电镜检测采自云南6个蔗区主栽品种上的28个甘蔗花叶病病样(分离物),其中25个病样的病叶汁液中观察到弯曲线状的病毒粒体,病叶组织中有风轮状和卷筒状内含体;对这25个分离物进行间接ELISA检测,16个与马铃薯Y病毒属抗血清呈阳性反应,其余呈阴性反应。根据蔗区及其主栽品种的不同,挑选7个分离物进行鉴别寄主测定,结果显示不同分离物鉴别寄主范围和致病性存在明显差异,分离物HH-1有范围最广的鉴别寄主和较强的致病性。克隆并测定HH-1基因组3'末端序列,序列分析发现HH-1的外壳蛋白(CP)基因共864个核苷酸,编码287个氨基酸,与高粱花叶病毒(Sorghum mosaic virus,SrMV)余杭分离物CP氨基酸序列的同源性最高,为97.7%;因此推定HH-1属于SrMV的一个新分离物。
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| 关 键 词： | 甘蔗花叶病 检测 高粱花叶病毒 |
| 文章编号： | 0412-0914（2007）03-0242-06 |
| 修稿时间： | 2006-03-082006-08-15 |
Detection of the viruses causing sugarcane mosaic disease and molecular identification of an isolate of them in Yunnan |
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| LI Wen-feng,DONG Jia-hong,DING Ming,FANG Qi,HUANG Ying-kun,SU Xiao-xia,LI Ting-ting,LUO Yan-qing,ZHANG Zhong-kai.Detection of the viruses causing sugarcane mosaic disease and molecular identification of an isolate of them in Yunnan[J].Acta Phytopathologica Sinica,2007,37(3):242-247. |
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| Authors: | LI Wen-feng DONG Jia-hong DING Ming FANG Qi HUANG Ying-kun SU Xiao-xia LI Ting-ting LUO Yan-qing ZHANG Zhong-kai |
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| Institution: | 1 Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661600, China;2 Yunnan Provincial Key Laboratory for Agro-Biotechnology, Kunming 650223, China |
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| Abstract: | Sugarcane mosaic disease has been occurring widely in Yunnan. 28 sugarcane mosaic disease samples from the leading varieties of six different growing areas were detected by electron microscope. Flexous fila-mentous particles were found in the sap of 25 isolates, pin-wheel and cylindrical inclusion bodies were produced in infected leaf tissues. These 25 isolates were detected by indirect ELISA, 16 ones could react to antibody of Potyvirus, but others couldn't. According to the sugarcane growing areas and local leading varieties, 7 isolates were selected to perform diagnostical host testing. The results showed diagnostical host range and pathogenicity of them were different, isolate HH-1 had the most extensive host range and strong pathogenicity. 3'terminal sequence of HH-1 genomic RNA was cloned and sequenced. The sequence analysis showed that coat protein(CP) gene of HH-1 contained 864 nucleotides, encoded 287 amino acids. It had a highest identity (97.7%) of amino acid sequence with Sorghum mosaic virus(SrMV), for which HH-1 was inferred as an isolate of SrMV. |
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| Keywords: | sugarcane mosaic disease detection Sorghum mosaic virus |
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