| 都匀毛尖茶挥发性成分及其抑菌活性测定 |
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| 引用本文： | 朱楠,陶晨,任竹君,张明,杨付梅,王道平,杨小生.都匀毛尖茶挥发性成分及其抑菌活性测定[J].甘肃农业大学学报,2016(3):127-134. |
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| 作者姓名： | 朱楠 陶晨 任竹君 张明 杨付梅 王道平 杨小生 |
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| 作者单位： | 1. 黔南民族医学高等专科学校化学教研室,都匀,558000;2. 贵州省中国科学院天然产物化学重点实验室,贵州 贵阳,550002 |
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| 基金项目： | 贵州省卫生厅项目(gzwkj2010-1-066)；贵州省卫生厅黔南民族医学高等专科学校科技联合基金项目(gzwkj2013-2-008)． |
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| 摘 要： | 【目的】比较不同生长阶段鲜叶和不同等级成品都匀毛尖茶挥发性成分的差异,并研究其抑菌活性.【方法】用同时蒸馏萃取法分别从3种都匀毛尖茶鲜叶和4种成品茶中提取挥发性成分,用气质联用仪进行测定,结合Nist2005和Wiley275谱库鉴定各成分,用峰面积归一化法测定各成分的相对含量.用琼脂平板打孔法将7个样品的挥发油对6种常见菌株进行抗菌试验.【结果】7个样品共检出124种挥发性化学成分,相同成分18种.3种鲜叶茶共检出73种挥发性成分,相同成分40种.4种成品茶共检出93种挥发性成分,相同成分35种.7个样品对枯草芽孢杆菌均有抑制作用.除一芽一叶初展外,其余6个样品对金黄色葡萄球菌均有抑制作用.【结论】加工对都匀毛尖茶的挥发性成分有一定影响,不同生长阶段、不同等级茶的挥发性成分也有差异.都匀毛尖茶挥发性成分具有一定的抑菌活性.
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| 关 键 词： | 都匀毛尖茶 挥发性成分 抑菌活性 蒸馏萃取法 气相色谱-质谱 |
Determination of volatile components and in vitro antibacterial activity of Duyun Maoj ian tea |
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| Abstract: | Obj ective]Comparing the difference in volatile components in fresh leaves at different growth stages and products of Duyun Maoj ian tea among different grades,and researching its antibacterial activity.Method]The volatile components of fresh leaves of 3 kinds of Duyun Maoj ian tea and 4 kinds of processed tea were extracted by SDE,and analyzed by GC-MS and various components were identified by spectral library Nist2005 and Wiley275,the relative content of each component was determined by peak are-a normalization method.The AGAR plate method of drilling hole was used to determine the antibacterial activities of the volatile oil of 7 samples against 6 bacterial species.Result]124 kinds of volatile chemical components were detected from the 7 samples in which 18 kinds were the 14same.73 kinds of volatile compo-nents were detected from 3 kinds of fresh tea leaves and 40 kinds were the same ingredients.93 kinds of volatile components were detected from 4 kinds of processed tea and 3 5 kinds were the same.7 samples had inhibitory effect on Bacillus subtilis.Except one bud and one leaf early development,the other 6 samples had inhibiting effect on Staphylococcus aureus.Conclusion]The processing of Duyun Maoj ian tea had certain influence on volatile compo-nents,volatile components differed at different growth stages and in different grades of processed tea.The volatile components of Duyun Maoj ian tea had certain antibacterial activity. |
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| Keywords: | Duyun Maoj ian tea volatile components antibacterial activity SDE GC-MS |
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