| 大蒜微型鳞茎诱导培养基的筛选初报 |
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| 引用本文： | 王云,蒲建刚,王德贤,常德昌,王琰,缑建民,葛亮.大蒜微型鳞茎诱导培养基的筛选初报[J].甘肃农业科技,2016(6):21-23. |
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| 作者姓名： | 王云 蒲建刚 王德贤 常德昌 王琰 缑建民 葛亮 |
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| 作者单位： | 1. 甘肃省天水市农业科学研究所,甘肃 天水,741001;2. 甘肃省天水市果树研究所,甘肃 天水,741002 |
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| 基金项目： | 甘肃省科技支撑计划项目“陇东南大蒜脱毒快繁技术研究及示范”(1204NKCE105) |
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| 摘 要： | 以吉木萨尔白蒜为试验材料，利用大蒜鳞茎盘为外植体，在B5+6-BA0.5 mg/L+NAA0.5 mg/L+蔗糖30 g/L+琼脂4.5 g/L培养基进行初始培养获得绿芽，在此基础上开展试管内诱导微鳞茎培养基配方研究。结果表明，1/2 MS＋IBA 1.5 mg/L＋NAA 0.1 gm/L＋蔗糖50 g/L＋琼脂4.5 g/L为组培苗试管内诱导鳞茎较好的培养基配方，在该培养基上大蒜绿芽第45天开始形成鳞茎，第90天鳞茎直径为0.3～0.5 cm。
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| 关 键 词： | 组织培养 鳞茎盘 诱导 培养基 大蒜 |
A Preliminary Report on Screen the Induction Medium of Garlic Micro Bulb |
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| WANG Yun,PU Jiangang,WANG Dexian,CHANG Dechang,WANG Yan,GOU Jian min,GE Liang.A Preliminary Report on Screen the Induction Medium of Garlic Micro Bulb[J].Gansu Agricultural Science and Technology,2016(6):21-23. |
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| Authors: | WANG Yun PU Jiangang WANG Dexian CHANG Dechang WANG Yan GOU Jian min GE Liang |
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| Abstract: | The Jimsar white garlic as experimental materials, using garlic bulb as explants, Initial culture is carried out to obtain green bud in B5+6-BA 0.5 mg/ L +NAA0.5 mg/L + sucrose 30 g/L + agar 4.5 g/L medium, on the basis of this study, the formula of the culture medium for inducing the micro bulb in vitro is developed. The result shows that 1/2 MS+IBA 1.5 mg/L+NAA 0.1 gm/L 1/2 MS + IBA 1.5 mg/L + sugar + 50 g/L + agar 4.5 g/L is a better culture medium formula for inducing bulb in tissue culture plantlet, the garlic green shoots began forming a bulb the in 45th day, the bulb diameter up to 0.3~0.5 cm in 90th day under the culture medium. |
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| Keywords: | Tissue culture Bulb plate Induction Culture medium Garlic |
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