| 甜瓜果实酸性转化酶基因cDNA片段的克隆 |
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| 引用本文： | 于喜艳,赵双宜,何启伟,孔庆国.甜瓜果实酸性转化酶基因cDNA片段的克隆[J].园艺学报,2003,30(3):346-348. |
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| 作者姓名： | 于喜艳 赵双宜 何启伟 孔庆国 |
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| 作者单位： | (山东农业大学园艺学院，泰安271018; 山东大学生命科学学院，济南250100; 山东省农业科学院蔬菜研究所，济南250100; 山东滨州职业学院，滨州256624) |
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| 摘 要： | 根据在GenBank中登录的番茄、胡萝卜和柑桔等酸性转化酶基因的保守序列设计引物，采用RT-PCR方法，从甜瓜果实总RNA中扩增出目标cDNA片段，克隆到pMD18-T载体中。序列分析表明，它与其它植物的酸性转化酶基因的同源性很高，与番茄氨基酸序列同源性为99％，说明已经成功克隆到甜瓜果实酸性转化酶基因cDNA片段，在GenBank中登记号AF490425。
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| 关 键 词： | 甜瓜 果实 RT-PCR 酸性转化酶基因 |
| 文章编号： | 0513-353X(2003)03-0346-03 |
| 收稿时间： | 2002-7-16 |
| 修稿时间： | 2002年7月16日 |
Cloning of a cDNA Fragment of Acid Invertase Gene from Cucumis melo L.Fruit |
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| Yu Xiyan ,Zhao Shuangyi ,He Qiwei ,and Kong Qingguo.Cloning of a cDNA Fragment of Acid Invertase Gene from Cucumis melo L.Fruit[J].Acta Horticulturae Sinica,2003,30(3):346-348. |
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| Authors: | Yu Xiyan Zhao Shuangyi He Qiwei and Kong Qingguo |
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| Institution: | ( School of Horticulture Shandong Agricultural University, Tai'an 271018, China; School of Sciences，Shandong University, Ji'nan 250100，China; Vegetable Institute，Shandong Academy of Agricultural Sciences，Ji'nan 250100，China; Binzhou Vocational College of Shandong Province，Binzhou 256624，China) |
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| Abstract: | PCR primers were designed based on the consensus domain of some acid invertase genes in GenBank.The aimed cDNA fragment was amplified from the total RNA isolated from melon fruit via RT-PCR and cloned into pMD18-T vector.The amino acid sequence determined by this fragment shows 99% homology to that of the corresponding region of acid invertase of tomato.This confirms that we have cloned a cDNA fragment from melon fruit successfully,the access number of this gene in GenBank is AF490425. |
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| Keywords: | Melon Fruit RT-PCR Acid invertase gene |
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