| 东北红豆杉中紫杉醇及三尖杉宁碱含量测定方法的研究 |
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| 引用本文： | 郭娜,周志强,王洋.东北红豆杉中紫杉醇及三尖杉宁碱含量测定方法的研究[J].林业科技,2009,34(3):62-64. |
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| 作者姓名： | 郭娜 周志强 王洋 |
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| 作者单位： | 东北林业大学生命科学学院,黑龙江哈尔滨,150040 |
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| 基金项目： | 黑龙江省博士后启动基金 |
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| 摘 要： | 采用均匀设计的方法，在不同条件下对东北红豆杉中紫杉醇及三尖杉宁碱进行提取，结果表明：室温下，80％甲醇超声波辅助提取30min为样品最佳提取条件；利用高效液相色谱仪对东北红豆杉不同部位中紫杉醇和三尖杉宁碱含量进行测定，其含量排序为：树皮〉当年生枝〉当年生叶〉假种皮，与树皮相比，虽然当年生枝叶中紫杉醇的含量低，但作为可更新资源，具有替代树皮成为提取紫杉醇原料的潜力。
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| 关 键 词： | 东北红豆杉 紫杉醇 三尖杉宁碱 超声波辅助提取 高效液相色谱法 |
Study on the Determination Method and Contents of Paclitaxel and Cephalomannine of Taxus cuspidata |
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| GUO Na.Study on the Determination Method and Contents of Paclitaxel and Cephalomannine of Taxus cuspidata[J].Forestry Science & Technology,2009,34(3):62-64. |
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| Authors: | GUO Na |
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| Institution: | Northeast Forestry University;Heilongjiang Harbin 150040 |
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| Abstract: | Paclitaxel and cephalomannine extraction from Taxus cuspidata were extracted by using Uniform design.The results showed that ultrasonically extracted by 80% methanol at room temperature for 30min were the optimum conditions.Paclitaxel and cephalomannine contents in different parts were determined,the order showed that bark>current-year branches>current-year leaves>aril.Although the effective components were lower in the current-year branches and leaves compared to the bark,as the regenerative resources it m... |
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| Keywords: | Taxus cuspidata Paclitaxel Cephalomannine Ultrasonic extraction method HPLC |
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