Proteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): Partial characterization and variations |
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| Authors: | M Loir C Labbé G Maisse A Pinson G Boulard B Mourot F Chambeyron |
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| Institution: | (1) Physiologie des Poissons, INRA, Campus de Beaulieu, 35042 Rennes, France |
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| Abstract: | The protein composition of seminal fluid, blood serum, sperm plasma membrane and flagellum of rainbow trout were analysed
by SDS-polyacrylamide gel electrophoresis. Immunological identity between proteins of the 2 fluids and sperm components was
studied using crossed immunoelectrophoresis, rocket immunoelectrophoresis and immunoblotting. Results indicate that many seminal
proteins are antigenically-related to serum proteins, proteins of sperm origin are present in seminal fluid in varying amounts,
depending on the animals and sampling time, and several serum-like seminal proteins are bound to spermatozoa.
Lipoproteins were isolated from seminal fluid (mean level: 33 μg/ml) and characterized. They were identified as being HDL-like
lipoproteins. A possible physiological role is proposed for these seminal lipoproteins. |
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| Keywords: | salmonid sperm seminal fluid membrane protein electrophoresis immunoblot lipoprotein |
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