| 棉铃虫蛋白酶体β5基因Habeta5的克隆与序列分析(英文) |
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| 引用本文： | 王更先,姜振,孔令斐,徐丽,司马杨虎,徐世清.棉铃虫蛋白酶体β5基因Habeta5的克隆与序列分析(英文)[J].农业科学与技术,2008,9(6):27-30. |
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| 作者姓名： | 王更先 姜振 孔令斐 徐丽 司马杨虎 徐世清 |
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| 作者单位： | 苏州大学现代丝绸国家工程实验室,苏州大学基础医学与生物科学学院,江苏苏州,215123 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | Objective] Using molecular biotechnology to clone the proteasome β5 gene from cotton bollworm (Helicoverpa armigera), this research aimed to provide basis for further research on the function of proteasome β5 gene in cotton bollworm. Method] Total RNA was extracted from midgut of cotton bollworm. The full length cDNA of Habeta5 gene was cloned by using rapid amplification of cDNA ends (RACE) technology, then sequence analysis was carried out. Result] The full length cDNA sequence was successfully cloned and isolated, named as Habeta5. It was 947 bp in length, contained an ORF (843 bp) and encoded 280 amino acid residues, with the predicted mass of 30.87 kD and isoelectric point(pI) of 9.60. In the deduced amino acid sequence, a proteasome β5 subunit domain lies between 74th to 261st amino acid residues. It has more than 62% identity to other insects such as Drosophila melanogaster. The proteasome β5 subunit conservative regions were very similar with each other. Molecular evolution by Neighbor Joining method indicated that Habeta5 was homologous with other proteasome β5 subunit of species. Conclusion] Sequence alignment shows that the cloned fragment is a proteasome β5 subunit gene (GenBank accession number: FJ358434).
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| 关 键 词： | Helicoverpa armigera Proteasome Cloning Habeta5 gene RACE |
Cloning and Sequence Analysis of Proteasome β5 Fragment in Helicoverpa armigera |
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| WANG Geng-Xian,JIANG Zhen,KONG Ling-fei,XU Li,SIMA Yang-hu,XU Shi-qing.Cloning and Sequence Analysis of Proteasome β5 Fragment in Helicoverpa armigera[J].Agricultural Science ＆ Technology,2008,9(6):27-30. |
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| Authors: | WANG Geng-Xian JIANG Zhen KONG Ling-fei XU Li SIMA Yang-hu XU Shi-qing |
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| Institution: | State Key Laboratory of Modern Silk, College of Basic Medicine and Biological Sciences, Soochow University, Suzhou 215153 |
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| Abstract: | |
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| Keywords: | Helicoverpa armigera Proteasome Cloning Habeta5 gene RACE |
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