| 应用DGGE技术比较池塘人工生物膜与水体微生物多样性差异 |
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| 引用本文： | 蒋建文,林文辉,王亚军,石存斌,潘厚军,吴淑勤.应用DGGE技术比较池塘人工生物膜与水体微生物多样性差异[J].安徽农业科学,2008,36(21). |
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| 作者姓名： | 蒋建文 林文辉 王亚军 石存斌 潘厚军 吴淑勤 |
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| 作者单位： | 中国水产科学院珠江水产研究所,广东广州510380;上海水产大学生命与科技学院,上海200090;中国水产科学院珠江水产研究所,广东广州,510380 |
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| 摘 要： | 目的]为改善养殖的微生态环境提供参考。方法]以无纺布为载体材料,采用PCR-DGGE技术比较了生物膜和水体微生物多样性的差异。结果]电泳分析表明,总共检测到46条不同的条带。第4天,生物基与水体样品的条带数差异不明显。第8天时,二者出现了不同条带。在10~18天,生物膜多样性指数一直低于水体,直到第18~20天才开始高于水体。第12天时生物膜上优势菌种普遍存在,但是部分开始出现衰减和增强的趋势。Band1、5、6分别是生物膜上第8、12、16天出现的特征性条带,除假单胞菌属外,其他结果均为未得到分离培养或者未经过命名的菌类。水样中的特征条带Band3、4同爱德华菌、弧菌属的相似性分别为94%、87%。结论]生物基载体可以改善池塘生物多样性。
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| 关 键 词： | 养殖池塘 微生态 生物基 生物膜 |
Comparison on The Difference of Microbial Diversity between Artificial Biofilm and Water in Pond by DGGE Technique |
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| JIANG Jian-wen et al.Comparison on The Difference of Microbial Diversity between Artificial Biofilm and Water in Pond by DGGE Technique[J].Journal of Anhui Agricultural Sciences,2008,36(21). |
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| Authors: | JIANG Jian-wen |
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| Abstract: | Objective] The study was to provide a base to improve the microecology environment of aquaculture. Method] With non-woven fabric as carrier material, the difference of microbial diversity of biomembrane and water body and biology base in culture pond was compared by PCR-DGGE technique. Result] Sum of 46 bands were detected by PCR-DGGE. On 4th day, the bands of biology base samples were not different from that of water samples. At 8th day, different bands were emerged among them. On 10th～18th day, the diversity index of biomembrane was lower than that of water and higher than that of water till 18～20 day. On 12th day, the dominant strains in biomembrane existed commonly, but part had a thread of decreasing or increasing. Band 1, 5, 6 on biomembrane was the characteristic bands on 8, 12, 16 day resp. Beside Pseudomonas lubricans, others were all fungoid without cultured or named. The characteristic band 3, 4 in water samples had 94% and 87% similarity with Edwardsiella ictaluri and Vibrio resp.. Conclusion] The carrier of biology base could improve the biology diversity in culture pond. |
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| Keywords: | Culture pond Microecology Biology base Biomembrane |
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