| 九种农药制剂和一种叶面肥中有机磷和氨基甲酸酯类隐性成分的快速检测 |
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| 引用本文： | 苍涛,汪志威,王新全,王祥云,吴声敢,吴长兴,陈丽萍,赵学平.九种农药制剂和一种叶面肥中有机磷和氨基甲酸酯类隐性成分的快速检测[J].农药学学报,2016,18(2):227-231. |
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| 作者姓名： | 苍涛 汪志威 王新全 王祥云 吴声敢 吴长兴 陈丽萍 赵学平 |
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| 作者单位： | 1.省部共建国家重点实验室培育基地"浙江省植物有害生物防控重点实验室", 农业部农药残留检测重点实验室, 浙江省农业科学院 农产品质量标准研究所, 杭州 310021 |
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| 基金项目： | 公益性行业(农业)科研专项经费项目(201303088);浙江省科技计划项目(2014C0200). |
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| 摘 要： | 建立了一种针对9种农药制剂和1种叶面肥中隐性添加23种有机磷或氨基甲酸酯类农药的快速检测方法。将农药制剂和叶面肥用磷酸盐缓冲溶液稀释静置后取上清液,加入乙酰胆碱酯酶溶液和显色剂,摇匀后反应5 min,加入底物后通过农药残留速测仪在波长412 nm处测定0~3 min的吸光度变化值。此外,对氧乐果、甲拌磷和甲基对硫磷在9种农药制剂和1种叶面肥中的检出限进行了验证。结果表明:该方法检出限在0.03~4.4 mg/L之间,当在9种农药制剂和1种叶面肥中的添加水平略低于检出限时,其抑制率均50%(为56%~100%)。本方法可用于定性判断农药制剂中是否添加了上述隐性成分。
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| 关 键 词： | 农药制剂 叶面肥 有机磷 氨基甲酸酯 隐性成分 快速检测 |
| 收稿时间： | 2015/11/9 0:00:00 |
Fast identification method of organophosphorus and carbamate in 9 kinds of pesticide formulations and 1 kind of leaf fertillizer |
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| CANG Tao,WANG Zhiwei,WANG Xinquan,WANG Xiangyun,WU Shenggan,WU Changxing,CHEN Liping and ZHAO Xueping.Fast identification method of organophosphorus and carbamate in 9 kinds of pesticide formulations and 1 kind of leaf fertillizer[J].Chinese Journal of Pesticide Science,2016,18(2):227-231. |
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| Authors: | CANG Tao WANG Zhiwei WANG Xinquan WANG Xiangyun WU Shenggan WU Changxing CHEN Liping and ZHAO Xueping |
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| Institution: | 1.State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | A simple and rapid method was developed to detect 23 kinds of organophosphorus or carbamates pesticides which were illegally added in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer. The pesticide formulations or leaf fertilizer were diluted with phosphate buffered saline solution and the supernatant was transferred into another test tube where the cholinesterase and chromogenic reagent were added orderly. This mixture was stirred and reacted for 5 min. The final detection was performed by a pesticide residue rapid measuring instrument which is used to measure the absorbance change of the mixture during 0-3 min at the wavelength of 412 nm after the substrate was added. Additionally, the limits of detection of omethoate, phorate and methyl parathion in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizers were verified. It was concluded that the limits of determination were ranged from 0.03 mg/L to 4.4 mg/L. The inhibition rates are <50%(0-26%) when the application levels in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer are marginally lower than the limit of detection. And they are >50%(56%-100%) when the application levels in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer are close to or marginally higher than the limit of detection. In conclusion, the hidden compositions mentioned above which are illegally added in pesticide formulations or leaf fertilizer could be qualitatively determined by the present method. |
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| Keywords: | pesticide formulation leaf fertilizer organophosphorus carbamate hidden components fast detection |
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