| 预处理及表面活性剂对米曲霉固体发酵产纤维素酶影响的研究 |
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| 引用本文： | 何海燕,陆秀青,秦文芳,何雪颖,韦秋艳,覃拥灵.预处理及表面活性剂对米曲霉固体发酵产纤维素酶影响的研究[J].中国饲料,2019(9):45-48. |
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| 作者姓名： | 何海燕 陆秀青 秦文芳 何雪颖 韦秋艳 覃拥灵 |
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| 作者单位： | 河池学院;微生物及植物资源开发利用广西高校重点实验室;广西壮族自治区粮油质量检验站 |
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| 基金项目： | 国家自然科学基金(31660017);河池学院高层次人才科研启动费项目(XJ2018GKQ020) |
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| 摘 要： | 本试验以米曲霉HML366为出发菌株,研究纤维素预处理及化学表面活性剂Tween 80对米曲霉固体发酵产纤维素酶的影响。结果表明:加入不同浓度的Tween 80对预处理过的蔗渣滤纸酶活的影响较为显著,蔗渣经5%氨水、5%乙酸、10%乙酸处理后,添加0.8%Tween 80的滤纸酶活分别可以达到6.6、6.3 U/mL和6.1 U/mL。当Tween 80浓度增加到1.2%浓度时,滤纸酶活有所下降。预处理过的蔗渣及添加Tween 80经发酵后,其发酵液中蛋白质含量较高,酶活较高。
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| 关 键 词： | 蔗渣纤维素 Tween80 酸碱预处理 滤纸酶活 |
Effect of pretreatment and surfactant on cellulase production by Aspergillus oryzae in solid fermentation |
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| HE Haiyan,LU Xiuqing,QIN Wenfang,HE Xueying,WEI Qiuyan,QIN Yongling.Effect of pretreatment and surfactant on cellulase production by Aspergillus oryzae in solid fermentation[J].China Feed,2019(9):45-48. |
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| Authors: | HE Haiyan LU Xiuqing QIN Wenfang HE Xueying WEI Qiuyan QIN Yongling |
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| Institution: | (College of Chemistry and Biological Engineering,Hechi University,Yizhou,Guangxi 546300,China;Guangxi Colleges Universities Key Laboratary of Exploitation and Utilization of Microbial and Botanical Resources,Yizhou,Guangxi 546300,China;Guangxi Zhuang Autonomous Region Grain and Oil Quality Inspection Station,Nanning,Guangxi 530031,China) |
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| Abstract: | HE Haiyan;LU Xiuqing;QIN Wenfang;HE Xueying;WEI Qiuyan;QIN Yongling(College of Chemistry and Biological Engineering,Hechi University,Yizhou,Guangxi 546300,China;Guangxi Colleges Universities Key Laboratary of Exploitation and Utilization of Microbial and Botanical Resources,Yizhou,Guangxi 546300,China;Guangxi Zhuang Autonomous Region Grain and Oil Quality Inspection Station,Nanning,Guangxi 530031,China) |
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| Keywords: | bagasse cellulose Tween 80 acid-base pretreatment filter paper enzyme activity |
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