| 航天诱变黑曲霉ZM-8菌株固态发酵产β-葡萄糖苷酶的研究 |
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| 引用本文： | 刘星斌,张宗舟,蔺海明,马旭光.航天诱变黑曲霉ZM-8菌株固态发酵产β-葡萄糖苷酶的研究[J].甘肃农业大学学报,2009,44(2). |
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| 作者姓名： | 刘星斌 张宗舟 蔺海明 马旭光 |
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| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070
2. 天水师范学院生命科学与化学学院,甘肃,天水,741000 |
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| 摘 要： | 以小麦秸秆和麸皮为原料,利用固态发酵对航天诱变后筛选的黑曲霉ZM-8菌株生产β-葡萄糖苷酶的条件和酶学特性进行了研究.结果表明,ZM-8菌株最佳培养基配方为:小麦秸秆粉与麸皮质量比为8∶2,氮源为4%的(NH4)2SO4,含水量200%;最佳的培养条件为:接种量为6%;初始pH值为6.5;培养温度为28℃;培养时间为144 h.在以上的培养基和培养条件下ZM-8菌株的β-葡萄糖苷酶酶活达到21.74 U/g,约为出发菌种的2.24倍.酶学实验表明,β-葡萄糖苷酶最适作用温度50℃,最适作用pH 5.0.
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| 关 键 词： | 黑曲霉 β-葡萄糖苷酶 酶学特性 |
Production of β-glucosidase by the space mutant strain Aspergillus niger ZM-8 on solid fermentation |
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| LIU Xing-bin,ZHANG Zong-zhou,LIN Hai-ming,MA Xu-guang.Production of β-glucosidase by the space mutant strain Aspergillus niger ZM-8 on solid fermentation[J].Journal of Gansu Agricultural University,2009,44(2). |
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| Authors: | LIU Xing-bin ZHANG Zong-zhou LIN Hai-ming MA Xu-guang |
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| Institution: | LIU Xing-bin1,ZHANG Zong-zhou2,LIN Hai-ming,MA Xu-guang (1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China,2.College of Life Science and Chemical,Tianshui Normal University,Tianshui 740101,China) |
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| Abstract: | Using wheat stalk and wheat bran as materials,the conditions for the producing of β-glucosidase by the space mutant strain Aspergillus niger ZM-8 on solid was studied.The results indicated that the optimum components of medium for β-glucosidase production were as follows:the mass ratio for wheat stalk and wheat bran was 8∶2,the nitrogen source was(NH4)2SO4 and its content was 4 %,the water content was 200 %.The optimum culture conditions were as follow:the inoculation amount was 6 %,the initial pH was 6.5,the culture temperature was 28 ℃ and the culture time was 144 hours.Under above medium and conditions,the enzyme activity of β-glucosidase was 21.74 U/g and increased by 2.24 times as compared with the parent strain.The results of enzymology characteristic demonstrated that the optimum temperature was 50 ℃ and the optimum pH was 5.0. |
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| Keywords: | Aspergilus niger β-glucosidase enzymatic characteristic |
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