| 川西高原黑唇鼠兔乳酸脱氢酶C基因cDNA的克隆及序列分析 |
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| 引用本文： | 贺庆华,徐亚欧,郑玉才,陈锋,张文磊.川西高原黑唇鼠兔乳酸脱氢酶C基因cDNA的克隆及序列分析[J].安徽农业科学,2010,38(5):2270-2273. |
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| 作者姓名： | 贺庆华 徐亚欧 郑玉才 陈锋 张文磊 |
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| 作者单位： | 西南民族大学生命科学与技术学院,四川成都,610041;西南民族大学生命科学与技术学院,四川成都,610041;西南民族大学生命科学与技术学院,四川成都,610041;西南民族大学生命科学与技术学院,四川成都,610041;西南民族大学生命科学与技术学院,四川成都,610041 |
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| 基金项目： | 国家民委资助项目(08XN04);;四川省应用基础研究(2008JY0068)项目 |
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| 摘 要： | 目的]为研究以鼠兔LDH-C4为靶蛋白的节育型鼠药奠定基础。方法]采用简并引物PCR克隆得到黑唇鼠兔LDH-C基因的EST序列,再根据EST序列采用RACE技术克隆出基因的开放阅读框（ORF）全长,和3’UTR序列。结果]整个cDNA全长1 498 bp,其中ORF长996 bp,编码332个氨基酸,3’UTR长486 bp。ORF序列比对显示LDH-C基因在大的分类单位上均很保守。系统树分析显示黑唇鼠兔的LDH-C基因和灵长类及偶蹄目的遗传距离较近,和啮齿目较远。结论]成功的克隆得到了黑唇鼠兔的LDH-C基因的cDNA序列。
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| 关 键 词： | LDH-C基因 RACE EST 序列分析 |
Cloning and Sequence Analysis of Lactate Dehydrogenase C (LDH-C) Gene from Black-lipped Pika in Western Sichuan Plateau |
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| HE Qing-hua et al.Cloning and Sequence Analysis of Lactate Dehydrogenase C (LDH-C) Gene from Black-lipped Pika in Western Sichuan Plateau[J].Journal of Anhui Agricultural Sciences,2010,38(5):2270-2273. |
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| Authors: | HE Qing-hua |
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| Institution: | College of Life Science and Technology;Southwest University for Nationalities;Chengdu;Sichuan 610041 |
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| Abstract: | Objective] The aim was to lay a foundation for studying contraceptive rodenticide with LDH-C4 as target protein. Method] EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length of open reading frame (ORF) and 3′UTR sequence were cloned by RACE technique. Result] The full length of cDNA was 1 498 bp containing an ORF of 996 bp and a 3′UTR of 486 bp. The ORF encoded a polypeptide of 332 amino acids. The alignment of LDH-C gene ORF nucleotide sequences from different species showed that the gene was conserved even between large taxons. The phylogenic tree showed that black-lipped pika LDH-C was closer to primates and artiodactyla than to rodents. Conclusion] cDNA sequence of LDH-C gene from black-lipped pika was cloned successfully. |
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| Keywords: | LDH-C gene RACE EST Sequence analysis |
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