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杂交朱顶红鳞茎不定芽诱导研究
引用本文:娄晓鸣,周玉珍,孔贤,吕文涛,张文婧.杂交朱顶红鳞茎不定芽诱导研究[J].安徽农业科学,2009,37(34):16769-16770.
作者姓名:娄晓鸣  周玉珍  孔贤  吕文涛  张文婧
作者单位:苏州农业职业技术学院,江苏苏州,215008;苏州农业职业技术学院,江苏苏州,215008;苏州农业职业技术学院,江苏苏州,215008;苏州农业职业技术学院,江苏苏州,215008;苏州农业职业技术学院,江苏苏州,215008
基金项目:江苏省农业三项工程,江苏省"青蓝工程",苏州市科技发展计划项目,苏州农业职业技术学院院级课题 
摘    要:目的]寻求杂交朱顶红鳞茎不定芽诱导的最佳配方,以提高杂交朱顶红组培扩繁系数。方法]以杂交朱顶红鳞茎为外植体,探讨不定芽诱导的最佳部位、最佳激素浓度,并比较不同品种的杂交朱顶红对不定芽产生的影响。结果]朱顶红鳞茎诱导不定芽最适部位为鳞茎下部,苹果诱导频率为87.12%,杂交后代18号诱导频率为31.55%;不定芽发生最适培养基为MS+TDZ0.5mg/L+NAA0.1而g/L,无论是诱导频率还是每外植体诱导不定芽数均为最高,苹果诱导频率为100.0%,杂交后代18号诱导频率为70.1%;不同品种不定芽分化差异较大,杂交后代比国外引进品种更难诱导不定芽。结论]为朱顶红的种球国产化提供了强有力的技术支持。

关 键 词:杂交朱顶红  鳞茎  不定芽  诱导

Study on Adventitious Bud Induction of Hippeastrum hybridum Bulb
LOU Xiao-ming et al.Study on Adventitious Bud Induction of Hippeastrum hybridum Bulb[J].Journal of Anhui Agricultural Sciences,2009,37(34):16769-16770.
Authors:LOU Xiao-ming
Institution:LOU Xiao-ming et al(Suzhou Polytechnic Institute of Agriculture,Suzhou,Jiangsu 215008)
Abstract:Objective] The research aimed to find optimum formula for adventitious bud induction of Hippeastrum hybridum bulb and improve its tissue culture propagation coefficient.Method] With H.hybridum bulb as explant,the best part,hormone concentration of induction were discussed,and the effects of different species H.hybridum on adventitious were compared.Result] The optimum part was the lower of bulb.The frequency of apple induction was 87.12%,and filial generation No.18 was 31.55%.The optimum culture medium w...
Keywords:Hippeastrum hybridum  Bulb  Adventitious bud  Induction
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