| ‘罗田甜柿’胚乳培养获得十二倍体再生植株 |
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| 引用本文： | 陈绪中,罗正荣.‘罗田甜柿’胚乳培养获得十二倍体再生植株[J].园艺学报,2004,31(5):589-592. |
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| 作者姓名： | 陈绪中 罗正荣 |
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| 作者单位： | (华中农业大学园艺林学学院, 武汉430070) |
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| 基金项目： | 国家自然科学基金资助项目 (3 0 0 70 5 2 9)，高等学校博士学科点专项基金资助项目 (2 0 0 3 0 5 0 40 14 ) |
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| 摘 要： | 取‘罗田甜柿’自然授粉后约70 d 的胚乳, 采用不同细胞分裂素和生长素组合进行离体培养。结果表明: 以1/ 2MS (1/ 2N ) + ZT (玉米素) 1.1 mg/ L + 2,4-D 1.0 mg/ L + CH (水解酪蛋白) 1000mg/ L 诱导产生的愈伤组织易获得再生植株; 形态学和细胞学检测确认再生植株中存在2n=12x=180 的个体。该十二倍体植株对中国原产甜柿的遗传改良可能具有潜在的应用价值。
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| 关 键 词： | 柿 甜柿 胚乳培养 多倍体 十二倍体 再生植株 |
| 文章编号： | 0513-353X(2004)05-0589-04 |
| 收稿时间： | 2003-10-14 |
| 修稿时间： | 2004-2-20 |
Dodecaploid Plantlets Regenerated from Endosperm Culture of‘Luotiantianshi’Persimmon |
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| Chen Xuzhong and Luo Zhengrong.Dodecaploid Plantlets Regenerated from Endosperm Culture of‘Luotiantianshi’Persimmon[J].Acta Horticulturae Sinica,2004,31(5):589-592. |
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| Authors: | Chen Xuzhong and Luo Zhengrong |
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| Institution: | ( College of Horticulture and Forestry , Huazhong Agricultural University , Wuhan 430070 , China) |
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| Abstract: | Endosperms of‘Luotiantianshi’persimmon ( Diospyros kaki Thunb. ) were cultured with after different auxin and cytokinin concent ration combination open pollination 70 days. Result s showed that the 1/ 2MS (1/ 2N) medium contained 2,4-D 1.0 mg/ L + ZT 1.1 mg/ L + CH 1000 mg/ L was better forinducing callus to from regenerated plantlets , Stomatal characteristics、flow cytometric analysis and chromosome counting proved dodecaploid (2n=12x= 180) presence in those regenerated plantlets. The regenerated dodecaploid plants could be used as parental material in breeding program. |
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| Keywords: | Persimmon Nonastringent persimmon Endosperm culture Polyploid Dodecaploid Regenerated plantlet$$$$ |
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