| 枇杷RAPD扩增产物的不同电泳检测及其序列特征分析 |
| |
| 引用本文： | 张晓莹,钱剑林,王化坤,宋长年,张彦苹,房经贵.枇杷RAPD扩增产物的不同电泳检测及其序列特征分析[J].浙江农业学报,2010,22(5):570. |
| |
| 作者姓名： | 张晓莹 钱剑林 王化坤 宋长年 张彦苹 房经贵 |
| |
| 作者单位： | 1.南京农业大学 园艺学院,江苏 南京210095； ;2.江苏省太湖常绿果树中心,江苏 苏州 215107 |
| |
| 基金项目： | 2008年省农业科技自主创新资金项目 |
| |
| 摘 要： | 为了更好地将RAPD技术应用于枇杷品种资源的鉴定以及遗传基础的研究,在选用11个碱基引物以及严格筛选PCR退火温度的最新RAPD技术优化的基础上,以16个枇杷品种为试验材料,对琼脂糖凝胶以及聚丙烯酰胺凝胶(PAGE)电泳检测的RAPD PCR扩增产物效果进行比较。结果表明，聚丙烯酰胺凝胶(PAGE)电泳检测出的总谱带数以及多态性谱带数均高于琼脂糖凝胶。根据两种电泳系统获得的标记信息进行枇杷品种遗传聚类分析,结果表明PAGE电泳的分析结果与枇杷的分类情况更为一致。随机对挑选的38个片段进行克隆与测序，发现37个片段都是对应引物的RAPD扩增产物，其中有6条是编码蛋白的基因片段,说明RAPD不仅扩增基因组上的非编码蛋白序列,而且可以扩增编码蛋白的基因片段,是能够揭示枇杷品种间基因组信息异同的理想的DNA标记技术。
|
| 关 键 词： | 枇杷 RAPD 琼脂糖凝胶电泳 PAGE |
Analysis of different electrophoresis and sequence characteristics of RAPD amplification products of loquat |
| |
| ZHANG Xiao-ying,QIAN Jian-lin,WANG Hua-kun,SONG Chang-nian,ZHANG Yan-ping,FANG Jing-gui.Analysis of different electrophoresis and sequence characteristics of RAPD amplification products of loquat[J].Acta Agriculturae Zhejiangensis,2010,22(5):570. |
| |
| Authors: | ZHANG Xiao-ying QIAN Jian-lin WANG Hua-kun SONG Chang-nian ZHANG Yan-ping FANG Jing-gui |
| |
| Institution: | 1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2 Evergreen Tree Center of Taihu Lake in Jiangsu Province, Suzhou 215107, China |
| |
| Abstract: | In order to utilize RAPD technology in identification of loquat cultivars and genetic analysis, the RAPD fingerprints of 16 loquat cultivars from agarose and polyacrylamide gel electrophoreses were compared, which were carried out with latest optimization of RAPD technique in choosing 11 nt primers and strict screening PCR annealing temperature. The results showed that more bands and more polymorphic bands could be detected by the polyacrylamide gel electrophoresis (PAGE) than the agarose gel electrophoresis. Two polygenetic trees derived from the fingerprint data collected from two electrophoreses were constructed, showing that the result from PAGE electrophoresis was more consistent with the regular classification of these loquat cultivars by phenotypic traits. The results of cloning and sequencing of 38 PCR amplified fragments showed that 37 fragments were RAPD amplified products generated by the corresponding primers, among which 6 sequences were segments of protein-encoding genes, suggesting RAPD could amplify both protein-encoding and non-protein-encoding gene sequences, and could reveal the genetic variability of different loquat cultivars. |
| |
| Keywords: | loquat RAPD agarose gel electrophoresis PAGE |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《浙江农业学报》浏览原始摘要信息 |
| 点击此处可从《浙江农业学报》下载免费的PDF全文 |
|
