| 广西的尼里—拉菲水牛和摩拉水牛mtDNA D-loop区遗传多样性研究 |
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| 引用本文： | 李双,汤嘉玉,王自豪,黄光云,滕少花,曹艳红,雷初朝,孙俊丽.广西的尼里—拉菲水牛和摩拉水牛mtDNA D-loop区遗传多样性研究[J].中国牛业科学,2020,46(5):1-4. |
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| 作者姓名： | 李双 汤嘉玉 王自豪 黄光云 滕少花 曹艳红 雷初朝 孙俊丽 |
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| 作者单位： | 广西壮族自治区畜牧研究所,广西家畜遗传改良重点实验室,广西南宁530001;西北农林科技大学动物科技学院,陕西杨凌712100;广西壮族自治区畜牧研究所,广西家畜遗传改良重点实验室,广西南宁530001;西北农林科技大学动物科技学院,陕西杨凌712100 |
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| 基金项目： | 广西创新驱动发展专项“广西畜禽种质资源的收集、评价与鉴定”（合同编号：桂科AA17204024），国家肉牛牦牛产业技术体系（CARS-37） |
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| 摘 要： | 目的]探究引入广西南宁的尼里-拉菲水牛和摩拉水牛的mtDNA D-loop区遗传多样性与母系起源。方法]采用PCR扩增、测序及生物信息学方法。结果]对从广西南宁采集的52个江河型水牛（尼里-拉菲水牛25个，摩拉水牛27个）mtDNA D-loop序列与GenBank下载的20条尼里-拉菲水牛和23条摩拉水牛序列进行联合分析，共检测到112个变异位点，定义42个单倍型，发现摩拉水牛（Hd: 0.934±0.027）与尼里-拉菲水牛的遗传多样性（Hd: 0.929±0.017）都很丰富。NJ系统发育树显示尼里-拉菲水牛和摩拉水牛含有江河型和沼泽型水牛mtDNA支系，表明尼里-拉菲水牛和摩拉水牛引入中国后均与沼泽型水牛进行了杂交，在外貌上很难区分。结论]引入广西南宁的尼里-拉菲水牛和摩拉水牛与沼泽型水牛存在广泛的血缘混杂现象。
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| 关 键 词： | 尼里-拉菲水牛 摩拉水牛 mtDNA D-loop 遗传多样性 |
| 收稿时间： | 2020/7/3 0:00:00 |
| 修稿时间： | 2020/8/20 0:00:00 |
Genetic Diversity of mtDNA D-loop Region of Nili-Ravi and Murrah Buffaloes in Guangxi Province |
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| Li Shuang,and Sun Junli.Genetic Diversity of mtDNA D-loop Region of Nili-Ravi and Murrah Buffaloes in Guangxi Province[J].China Cattle Science,2020,46(5):1-4. |
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| Authors: | Li Shuang and Sun Junli |
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| Abstract: | Objective] To analyze the genetic diversity and maternal origin of the introduced Nili-Ravi and Murrah buffalo in Nanning city, Guangxi Province. Method] PCR, sequencing and Bioinformatics were used. Result] The complete mtDNA D-loop regions of 52 introduced river buffaloes (including 25 Nili-Ravi and 27 Murrah buffaloes) in Nanning city, Guangxi Province and 20 Nili-Ravi and 23 Murrah buffaloes downloaded from GenBank were analyzed, a total of 112 polymorphic sites were detected, which constituted 42 haplotypes. The Murrah buffalo and Nili-Ravi buffaloes showed rich genetic diversity with Hd=0.934±0.027 and Hd=0.929±0.017, respectively. NJ tree showed that the Nili-Ravi and Murrah buffalo included the mtDNA lineages of river buffalo and swamp buffalo, which demonstrated that the introduced Nili-Ravi and Murrah buffalo in China crossbred with native swamp buffaloes. Ii is very difficult to distinguish their appearance of Nili-Ravi and Murrah buffalo and their hybrids with swamp buffaloes. Conclusion] The introduced Nili-Ravi and Murrah buffaloes in Nanning city of Guangxi Province showed extensive introgression with swamp buffaloes. |
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| Keywords: | Nili-Ravi buffalo Murrah buffalo mtDNA D-loop genetic diversity |
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