In vitro grafting of cashew (Anacardium occidentale L.) |
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| Institution: | 1. Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Campus universitaire Tunis El Manar, Université de Tunis El Manar, 2092 Tunis, Tunisia;2. CNRS UMR 6270, Laboratoire Polymères, Biopolymères, Surfaces, University of Rouen, Mont Saint-Aignan, France;3. PISSARO Proteomic Platform, Mont Saint-Aignan, France;4. Montpellier SupAgro, UMR 1334, Amélioration Génétique et Adaptation des Plantes (AGAP), Montpellier, France;5. INRA/CBNMed, UMR AGAP, Montpellier, France;6. Cadi Ayyad University, Laboratoire de Biotechnologie de la Valorisation et la Protection des Agro-Ressources, Faculté des Sciences et Techniques de Marrakech, Morocco;7. Cadi Ayyad University, Faculté Polydisciplinaire de Safi, Département de Biologie, Sidi Bouzid, B.P. 4162, 46000 Safi, Morocco;1. College of Horticulture, Gansu Agricultural University, Lanzhou 730070, PR China;2. Department of Horticulture, FoA, University for Development Studies, P. O. Box TL 1882, Tamale, Ghana;1. Department of Horticultural Sciences, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran;2. Pistachio Research Station, Research Center of Agricultural and Natural Resources, Semnan Province, Damghan, Iran;3. Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Arak University, 38156-8-8349 Arak, Iran;4. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran;1. Department of Home and Health Sciences, Allama Iqbal Open University Islamabad, Pakistan;2. Department of Pharmacology, University of Health Sciences Lahore, Pakistan;3. Arid Agriculture University, Rawalpindi, Pakistan;4. Department of Botany, University of Baluchistan, Quetta, Pakistan;5. Experimental Animal Laboratory, University of Health Sciences Lahore, Pakistan |
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| Abstract: | A successful micrografting technique in cashew was developed using in vitro germinated seedlings as rootstocks and axenic shoot cultures (shoot-tip and nodal cultures) established from mature tree source as microscions. In vitro germinated seedlings, which emerged 20–25 days after inoculation on absorbent cotton, were decapitated and used as rootstock. Mature tree explants initiated on hormone-free Murashige and Skoog Physiol. Plant. 15 (1962) 473] (MS) modified medium were made into scion of 3–15 mm length for grafting. Micrografts could be easily cultured on hormone-free liquid half-MS medium and were potted out after 10–12 weeks of culture growth. Grafting success was dependent on the method of grafting and size of the scion. Shoot-tip grafting and side grafting were equally successful (79.5–100%). Length of scion shoot had significant effect on micrografting success. Graft success was high (79.5%) when the scion length was >5 mm and it was less (0.5%) when size of scion was small (3–5 mm). Scion presoaked in either water or 0.01% ascorbic acid and 0.015% citric acid (1:1) reduced phenolic browning and drying of scion. Micrografting techniques standardized could be used for rejuvenation of shoot explants of mature tree. |
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