| PRV闽A株Bam HI片段克隆及其第7片段的鉴定 |
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| 引用本文： | 娄高明,郭万柱.PRV闽A株Bam HI片段克隆及其第7片段的鉴定[J].中国兽医学报,1996,16(2):108-112. |
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| 作者姓名： | 娄高明 郭万柱 |
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| 作者单位： | 解放军农牧大学生物工程实验室，四川农业大学动物科技学院，军事医学科学院生物工程所 |
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| 摘 要： | 用鸟枪法将ＰＲＶ闽Ａ株的ＢａｍＨＩ酶切片断克隆到ｐＢＲ３２２质粒中，再经抗性筛选、酶切鉴定和菌落原位杂交，证实已克隆了ＰＲＶ闽Ａ株１４个ＢａｍＨＩ酶切片段中的１２个，从而构建了其基因文库，通过Ｓｏｕｔｈｅｒｎ转印杂交和酶切图谱分析鉴定了重组质粒ｐＰＲ１２８，其插入片段含量包含了ＰＲＶ闽Ａ株糖蛋白ｇｐ５０基因在内的ＢａｍＨＩ－７片段，核酸长约６．８ｋｂ。
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| 关 键 词： | 伪狂犬病 病毒 闽A株 克隆 |
Identification of Bam HI-7 Fragment and Cloning of Bam HI Restriction Enzyme Fragments of Pseudorabies VirusMin-A Strain DNA |
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| Lou Gaoming,Fei Enge, Xuan Hua, Li Youmin.Identification of Bam HI-7 Fragment and Cloning of Bam HI Restriction Enzyme Fragments of Pseudorabies VirusMin-A Strain DNA[J].Chinese Journal of Veterinary Science,1996,16(2):108-112. |
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| Authors: | Lou Gaoming Fei Enge Xuan Hua Li Youmin |
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| Abstract: | Pseudoraties virtis(PRV)Min-A strain isolated first in China was prop-agated in MDBK cells and its DNA was purified.The Bam HI restriction enzyme frag-ments of PRV Min-A strain DNA digested completely by Bam HI were cloned into theplasmid pBR322.The Bam HI restriction enzyme fragments except Bam HI-1,2 werecloned by resistance screening, restriction enzyme analysis and in situ hybridization.The genomic library of PRV Min-A strain DNAs was constructed.The recombinantplasmid pPR128 inserted with Bam HI-7 fragment that was approximately 6.8 kb andcontained complete gp50 gene was identified by restriction enzyme analysis and Southernblot hvbridization. |
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| Keywords: | pseudoraties virus Min-A strain cloning Bam HI-7 fragment |
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