| 基于高光谱成像技术的菊花花色表型和色素成分分析 |
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| 引用本文： | 伏静,戴思兰.基于高光谱成像技术的菊花花色表型和色素成分分析[J].北京林业大学学报,2016,38(8):88-98. |
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| 作者姓名： | 伏静 戴思兰 |
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| 作者单位： | 北京林业大学园林学院,花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心 |
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| 基金项目： | 国家自然科学基金项目(31272192)。 |
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| 摘 要： | 为无损、高效测定菊花花色表型和色素成分及含量,以菊花160个品种为研究材料,用紫外分光光度法测定舌状花的色素种类和含量;用高光谱成像技术对菊花不同品种头状花序上不同花轮位置上舌状花不同部位的高光谱反射值进行测定;对高光谱反射指数与舌状花色素含量实测值间的相关性进行分析。结果表明:1)菊花头状花序中轮的舌状花正面的中部或偏后部获得的高光谱测定数据稳定。2)菊花相同色系品种具有相似的高光谱特征曲线,不同色系品种高光谱特征曲线存在差异。3)高光谱反射指数RRed/RGreen、ARI、mARI、mCRI、TCARI与菊花舌状花色素含量之间有较好的相关性,并利用80个品种分别建立了色素成分及含量监测模型。4)利用另外80个菊花品种进行验证发现,以RRed/RGreen、ARI和mARI建立的花青素含量监测模型、mCRI建立的类胡萝卜素含量监测模型和TCARI建立的叶绿素含量监测模型精度较高,可以无损、快速地测定菊花舌状花色素成分和含量。本研究为观赏植物花色表型分析和色素成分及含量的测定提供了一种新的思路和方法。
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| 关 键 词： | 菊花 花色表型 色素含量 高光谱反射指数 |
| 收稿时间： | 2015-12-07 |
Analysis of color phenotypic and pigment contents of chrysanthemum based on hyperspectral imaging |
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| FU Jing,DAI Si-lan.Analysis of color phenotypic and pigment contents of chrysanthemum based on hyperspectral imaging[J].Journal of Beijing Forestry University,2016,38(8):88-98. |
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| Authors: | FU Jing DAI Si-lan |
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| Institution: | Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China. |
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| Abstract: | To measure color phenotypic and pigment contents of chrysanthemum, we used 160 varieties of chrysanthemum as research materials. Pigment composition and contents were measured using UV spectrophotometry. Hyperspectral reflection values of different varieties and different parts of ray flowers and different flower rounds were measured by hyperspectral imaging technology. The relationship between hyperspectral reflection indices and pigment contents were analyzed using correlation analysis. The results showed that:1 ) Data measured in the middle or rear part of ray flowers in the middle of wheels were stable. 2 ) Varieties of the same color had similar hyperspectral characteristics. Different varieties of chrysanthemum had significant differences in hyperspectral characteristics. 3 ) Hyperspectral reflection values RRed/RGreen, ARI, mARI, mCRI and TCARI showed a close correlation with pigment contents of ray flowers and their monitoring models were established accordingly using 80 varieties. 4) Using another 80 varieties as the verification materials, we found that models established with RRed/RGreen, ARI and mARI for anthocyanin content monitoring, and models established with mCRI for carotenoid content monitoring and models established with TCARI for chlorophyll monitoring had high accuracy. The established models can be used for nondestructive and fast determination of pigment composition and contents of chrysanthemum. Our research provides new ideas and methods for color phenotypic analysis and measurement of pigment composition and content of ornamental plants. |
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| Keywords: | chrysanthemum color phenotypic pigment contents hyperspectral reflection index |
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