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Harpin处理对苹果梨黑斑病的抑制及抗性酶的诱导
引用本文:王军节,王毅,葛永红,毕阳.Harpin处理对苹果梨黑斑病的抑制及抗性酶的诱导[J].甘肃农业大学学报,2006,41(5):114-117.
作者姓名:王军节  王毅  葛永红  毕阳
作者单位:甘肃农业大学食品科学与工程学院,甘肃,兰州,730070
基金项目:甘肃省农委(9870599)项目
摘    要:研究了采后Harpin真空渗透处理对苹果梨(Pyrus bretschneideri‘Pingguo’)损伤接种互隔交链孢(Alternar-ia alternata)的影响及部分机理.结果表明:用50、100和150 mg/L的Harpin处理均可明显抑制损伤接种果实的发病率,其中以50 mg/L Harpin的效果最好.此外,该浓度处理还明显降低损伤接种果实的病斑面积.苹果梨经50 mg/LHarpin处理再用(A.alternata)挑战后果实体内的过氧化物酶(POD)、几丁质酶(CHT)和苯丙氨酸解氨酶(PAL)活性明显升高,酚类物质的含量也显著积累.由此表明,Harpin对苹果梨黑斑病有效抑制与诱导果实体内这3种抗性酶的活性以及提高酚类物质含量密切相关.

关 键 词:Harpin  苹果梨  互隔交链孢  采后病害
文章编号:1003-4315(2006)05-0114-04
收稿时间:2006-06-27
修稿时间:2006-06-27

Inhibiting effect of postharvest Harpin treatment on Alternaria rot and induction to resistance enzymes of Pyrus bretschneideri 'Pingguo'
WANG Jun-jie,WANG Yi,GE Yong-hong,BI Yang.Inhibiting effect of postharvest Harpin treatment on Alternaria rot and induction to resistance enzymes of Pyrus bretschneideri ''''Pingguo''''[J].Journal of Gansu Agricultural University,2006,41(5):114-117.
Authors:WANG Jun-jie  WANG Yi  GE Yong-hong  BI Yang
Institution:College of Food Science and Engineering, Gansu Agricultural of University, Lanzhou 730070,China
Abstract:The effect of vacuum infiltration treatment with Harpin on Alternaria rot of Pyrus bretschneideri'Pingguo' inoculated with Alternaria alternata and some resistance mechanism were studied.The results showed that Harpin treatment at the concentration levels of 50,100 and 150 mg/L significantly inhabited the disease incidence.The concentration at 50 mg/L showed the best inhibi-tion,and the same treatment also reduced the lesion area.The treatment at 50 mg/L increased the activities of peroxidase(POD),chitinase(CHT) and phenylalanine ammonia-lyase(PAL),and total phenolic content in the fruits challenged with A.alternata.It suggested that the effective inhibition of Harpin treatment on Alternaria rot in the pear cultivar was associated with the induction of the three enzyme activities and the enhancement of the total phenolic content in the fruits.
Keywords:Harpin  Pyrus bretschneideri'Pingguo'  Alternaria alternata  postharvest disease
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