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| 应用激光共聚焦显微技术研究Ca2+在三角帆蚌组织内的积累与分布 | |
| 引用本文： | 李文娟,施志仪,郝莹莹,叶显峰.应用激光共聚焦显微技术研究Ca2+在三角帆蚌组织内的积累与分布[J].水产学报,2011,35(2):214-220. |
| 作者姓名： | 李文娟 施志仪 郝莹莹 叶显峰 |
| 作者单位： | 1. 上海海洋大学农业部水产种质资源与养殖生态重点开放实验室,上海,201306 2. 上海海洋大学农业部水产种质资源与养殖生态重点开放实验室,上海,201306;上海市高校水产养殖学E-研究院,上海海洋大学水产与生命学院,上海,201306 3. 安徽省芜湖市繁昌县农委,安徽,繁昌,241200 |
| 基金项目： | 上海市博士后科研资助计划面上项目(09R21413200); 上海市科委基础重大项目(06aj14003); 上海市重点学科水生生物学建设项目资助(S30701) |
| 摘 要： | 为了探讨淡水贝类Ca2+吸收和转运信号传递机理,采用激光共聚焦技术研究了三角帆蚌不同组织细胞在静息状态下细胞内游离Ca2+浓度,以及水体Ca2+浓度对外套膜组织细胞内钙沉积的影响。试验选取10只健康的三角帆蚌,分别获得外套膜外膜、内膜、斧足、腮及内脏团上表皮组织细胞,短期培养后用Fluo-3/AM荧光探针孵育细胞1 h,观察细胞内Ca2+荧光强度。研究结果表明,蚌不同组织细胞内Ca2+荧光强度存在显著差异,外套膜外膜细胞内Ca2+荧光强度最高,内脏团上表皮细胞的最低(P<0.05);育珠三角帆蚌在相同Ca2+浓度的孵育水平下,外套膜细胞内Ca2+荧光强度比非育珠蚌都有增加的趋势,其中在1.25 mmol/L添加组中,两组外套膜内膜细胞内Ca2+荧光强度差异达到显著水平(P<0.05);不同Ca2+孵育水平对细胞内Ca2+荧光强度有显著影响(P<0.05),随着Ca2+在孵育液中浓度的升高,外套膜细胞内Ca2+荧光强度显著增强(P<0.05),表明外套膜是从外界吸收Ca2+的主要组织,蚌体珍珠的培育增强了其对Ca2+的沉积,并且水体Ca2+浓度在1.25～3.00 mmol/L有助于蚌体内Ca2+的贮藏,这对进... |
| 关 键 词： | 三角帆蚌 细胞培养 激光共聚焦技术 Ca2 沉积 |
| 收稿时间： | 7/18/2010 3:09:34 PM |
| 修稿时间： | 11/8/2010 9:25:59 PM |
Study of Ca2+ deposition and distribution in cells from different tissues with laser scanning confocal microscopy in Hyriopsis cumingii |
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| LI Wen-juan,SHI Zhi-yi,HAO Ying-ying and YE Xian-feng.Study of Ca2+ deposition and distribution in cells from different tissues with laser scanning confocal microscopy in Hyriopsis cumingii[J].Journal of Fisheries of China,2011,35(2):214-220. | |
| Authors: | LI Wen-juan SHI Zhi-yi HAO Ying-ying and YE Xian-feng |
| Institution: | LI Wen-juan1,SHI Zhi-yi1,2,HAO Ying-ying1,YE Xian-feng3(1.Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystems,Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China,2.Aquaculture Division,E-Institute of Shanghai Municipal Education Commission,3.Agriculture Committee of Fanchang of Wuhu,Fanchang 241200,China) |
| Abstract: | The Ca2+metabolism is one of vital factors relative to growth of shell and pearl in freshwater mussel.In order to probe the Ca2+ absorption and transportation in freshwater shellfish,Ca2+ concentration of cells(under resting state)from different tissues and effect of Ca2+ concentration of culture medium on Ca2+deposits within the cells from outer mantle were researched by technology of laser scanning confocal microscopy in Hyriopsis cumingii.The total of healthy 10 H.cumingii with pearl and without pearl we... |
| Keywords: | Hyriopsis cumingii cell culture laser confocal technology Ca2 deposition |
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