|
|||||
|
|
| SSR-BSA技术对乌鳢性别差异标记的初步筛选 | |
| 引用本文： | 刘改艳,陈昆慈,郑光明,朱新平,赵建,徐鹏,孙效文.SSR-BSA技术对乌鳢性别差异标记的初步筛选[J].水产学报,2011,35(2):170-175. |
| 作者姓名： | 刘改艳 陈昆慈 郑光明 朱新平 赵建 徐鹏 孙效文 |
| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,广东,广州,510380;上海海洋大学水产与生命学院,上海,201306 2. 中国水产科学研究院珠江水产研究所,广东,广州,510380 3. 中国水产科学研究院,北京,100039 4. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070 |
| 基金项目： | 国家公益性行业(农业)科研专项(200903045) |
| 摘 要： | 采用SSR结合BSA技术对1个家系96个乌鳢个体(雌雄各48个)进行性别差异标记的筛选。首先构建了雌雄基因池(各24个个体),然后用140对微卫星引物对其进行扫描,发现3对引物(HLJWL17,HLJWL59,HLJWL70)在雌雄基因池间扩增出差异条带,且都出现在雌性基因池中。用构建基因池的48个个体对3对引物进行第一轮单个体验证,HLJWL17和HLJWL70在基因池中扩增出的差异条带仅在极个别个体中出现,而HLJWL59的差异条带在绝大多数的雌性个体中被成功扩增出来,雄性个体皆无。用其余的48个个体对HLJWL59进行第二轮单个体验证,得到同样结果。对HLJWL59在8个雌性个体中扩增出来的差异基因片段进行克隆并测序。将8个个体的测序结果用Vector 8.0进行多序列比对,证实各个个体得到的条带是同一序列,该序列长度为243 bp,以TGC为重复单位,GC含量为51%。通过BLASTn比对,发现在GenBank数据库中无同源性序列存在。经统计,此差异标记在雌性乌鳢中出现的概率为62.5%,即该标记对该家系乌鳢性别的正确判别率为81.25%。 |
| 关 键 词： | 乌鳢 微卫星 性别差异 混合分离群体法 |
| 收稿时间： | 9/14/2010 9:35:06 AM |
| 修稿时间： | 11/5/2010 3:40:02 PM |
Screening and identification of female-specific DNA fragments in Channa argus using SSR-BSA |
|
| LIU Gai-yan,CHEN Kun-ci,ZHENG Guang-ming,ZHU Xin-ping,ZHAO Jian,XU Peng and SUN Xiao-wen.Screening and identification of female-specific DNA fragments in Channa argus using SSR-BSA[J].Journal of Fisheries of China,2011,35(2):170-175. | |
| Authors: | LIU Gai-yan CHEN Kun-ci ZHENG Guang-ming ZHU Xin-ping ZHAO Jian XU Peng and SUN Xiao-wen |
| Institution: | LIU Gai-yan1,2,CHEN Kun-ci1,ZHENG Guang-ming1,ZHU Xin-ping1,ZHAO Jian1,XU Peng3,SUN Xiao-wen4(1.Pearl River Fisheries Research Institute(PRFRI),Chinese Academy of Fishery Sciences,Guangzhou 510380,China,2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,3.Chinese Academy of Fishery Sciences,Beijing 100039,4.Heilongjiang River Fisheries Research Institute,Harbin 150070,China) |
| Abstract: | Microsatellites combined with bulked segregation analysis(BSA)were used to screen the gender differences of 96 individuals(48 for male and female each)from a family of Channa argus.Two gene pools were constructed using 24 individuals for male and female each separately,gene pools were used to scan using totally 140 pairs of microsatellite primers and specific DNA fragments of female gene pool were amplified by 3 pairs of primers(HLJWL17,HLJWL59,HLJWL70).Then,the specific DNA fragments amplified by the three... |
| Keywords: | Channa argus simple sequence repeats gender-differences bulked segregation analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《水产学报》浏览原始摘要信息 | |
| 点击此处可从《水产学报》下载免费的PDF全文 | |