| 基于SSR标记的云南糯玉米、爆裂玉米地方种质遗传多样性研究 |
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| 引用本文： | 吴渝生,郑用琏,孙荣,伍少云,顾红波,毕有华.基于SSR标记的云南糯玉米、爆裂玉米地方种质遗传多样性研究[J].作物学报,2004,30(1):36-42. |
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| 作者姓名： | 吴渝生 郑用琏 孙荣 伍少云 顾红波 毕有华 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室，湖北武汉 430070 |
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| 基金项目： | 教育部西部地区高校高级访问学者计划项目 |
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| 摘 要： | 利用SSR标记分析了16份和14份代表云南不同生态地区糯玉米、爆裂玉米地方种的遗传多样性。从96对SSR引物中分别筛选出分布于玉米基因组10条染色体上的61对引物和43对引物，每对引物可以分别稳定地检测到1～12个和1～13个多态性片段，糯玉米共226个，平均为3.70个，片段大小介于70～700 bp之间；爆裂玉米共232个，平均检测的多
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| 关 键 词： | 云南 糯玉米 爆裂玉米 地方品种 遗传多样性 SSR标记 |
| 收稿时间： | 2002-12-16 |
| 修稿时间： | 2002年12月16 |
Genetic Diversity of Waxy Corn and Popcorn Landraces in Yunnan by SSR Markers |
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| WU Yu Sheng ,ZHENG Yong Lian ,SUN Rong ,WU Shao Yun ,GU Hong Bo ,BI You Hua.Genetic Diversity of Waxy Corn and Popcorn Landraces in Yunnan by SSR Markers[J].Acta Agronomica Sinica,2004,30(1):36-42. |
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| Authors: | WU Yu Sheng ZHENG Yong Lian SUN Rong WU Shao Yun GU Hong Bo BI You Hua |
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| Institution: | National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei |
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| Abstract: | waxy corn landraces and 14 popcorn landraces in Yunnan were analyzed by SSR marker for genetic diversity. 61 primers and 43 primers were screened to be specific with chromosome 10 of waxy corn and popcorn respectively from 96 primers tested. Polymorphic fragments were amplified stably by each specific primer, 1-12 for waxy corn and 1-13 for popcorn. In total 226 specific DNA bands of 70-700 bp in size were detected in waxy corn with an average of 3.70 fragments for each primer. 9 primers accounting for 14.7% in the primers used could detect 1 allele, 2 primers accounting for 3.3% could detect 11 and 12 alleles respectively. Other 50 primers accounting for 82.0% could normally detect 2-7 alleles. For popcorn landraces, 232 alleles of 65-490 bp in size were detected in total, 5.40 alleles by each primer on average. 3 primers accounting for 7.0% could detect 1 allele, other 3 primers accounting for 7.0% could detect 12 and 13 alleles respectively. The rest of 37 primers accounting for 86.0% could usually detect 2-9 alleles. The results suggested that higher genetic polymorphism was maintained in local waxy corn and popcorn landraces of Yunnan. The result by cluster analysis showed that waxy corn could be divided into 3 groups and 5 sub groups, and popcorn into 3 groups and 4 sub groups. That was generally in accordance with the tendency of topography and altitude in Yunnan. |
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| Keywords: | Yunnan Waxy corn Popcorn Landrace Genetic diversity SSR marker |
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