| 小尾寒羊、无角道赛特及其杂交后代BMPR-IB基因多态性研究 |
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| 引用本文： | 王红娜,张英杰,刘月琴,任立坤,杨少华.小尾寒羊、无角道赛特及其杂交后代BMPR-IB基因多态性研究[J].黑龙江畜牧兽医,2012(11):52-55. |
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| 作者姓名： | 王红娜 张英杰 刘月琴 任立坤 杨少华 |
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| 作者单位： | 河北农业大学动物科学技术学院 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2008BADB2B04-9);转基因生物新品种培育重大专项(2008ZX08008-003-01) |
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| 摘 要： | 为了检测骨骼形态发生蛋白IB型受体(BMPR-IB)基因在小尾寒羊母羊、无角道赛特公羊、道×寒F1和F2代杂交公羊及道×寒F2横交固定所产种公羊中的多态性,分析小尾寒羊基因型与产羔数的相关性,试验采用PCR-RFLP法对BMPR-IB基因进行分型。结果表明:在无角道赛特公羊中只有1种基因型,即野生型(++);在小尾寒羊母羊中检测到2种基因型(BB和B+),基因型频率分别为0.35,0.65;在道×寒F1和F2代杂交公羊中检测到2种基因型(B+和++),F1代基因型频率分别为0.75,0.25,F2代基因型频率分别为0.30,0.70。小尾寒羊的2种基因型产羔数差异极显著(P<0.01),BMPR-IB基因的遗传方式符合孟德尔遗传定律。
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| 关 键 词： | 绵羊 骨骼形态发生蛋白IB型受体(BMPR-IB)基因 多态性分析 产羔数 |
Study on polymorphism of BMPR-IB gene in small-tailed Han sheep, hornless Dorset Sheep and their hybrids |
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| WANG Hong-na,ZHANG Ying-jie,LIU Yue-qin,Ren Li-kun,YANG Shao-hua.Study on polymorphism of BMPR-IB gene in small-tailed Han sheep, hornless Dorset Sheep and their hybrids[J].Heilongjiang Animal Science And veterinary Medicine,2012(11):52-55. |
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| Authors: | WANG Hong-na ZHANG Ying-jie LIU Yue-qin Ren Li-kun YANG Shao-hua |
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| Institution: | (College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071000,China) |
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| Abstract: | To detect the polymorphism of BMPR-IB gene in small-tailed Han ewes,hornless Dorset rams,their F1,F2 hybrid rams and the rams produced by F2 crossing,analyzing the correlation of the genotype of Han sheep with litter size.The BMPR-IB gene was typed by the method of PCR-RFLP.The results showed that there was only one genotype(wild type,++) in hornless Dorset rams.In small-tailed Han sheep,2 kinds of genotypes including pure mutant type(BB)and heterozygous mutant type(B+) were detected,its genotype frequency were 0.35,0.65,respectively.In the F1 and F2 generations between Dorset sheep and small-tailed Han sheep,2 kinds of genotypes(B+and++) were detected,the genotype frequency of F1 were 0.75,0.25,respectively;The genotype frequency of F2 were 0.30,0.70,respectively.It is concluded that the litter size between 2 kinds of genotypes in small-tailed Han sheep has an extremely significant difference(P<0.01),and the inheritance mode of BMPR-IB gene is consistent with Mendelian inheritance patterns. |
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| Keywords: | sheep bone morphogenetic protein receptor IB(BMPR-IB) gene analysis of polymorphism litter size |
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