| Ag─NOR染色技术在豌豆染色体研究中的应用 |
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| 引用本文： | 李贵全,赵晓明,王玉庆.Ag─NOR染色技术在豌豆染色体研究中的应用[J].山西农业大学学报(自然科学版),1995(1). |
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| 作者姓名： | 李贵全 赵晓明 王玉庆 |
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| 作者单位： | 山西农业大学农学系 |
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| 摘 要： | 本文以山西省栽培的硬粒豌豆品种为实验材料，对豌豆个体之间细胞核仁的数目Ａｇ—ＮＯＲ的位置变化，及在实验过程中所观察到的染色体分化程度进行了研究。结果表明，该品种在问期核可见到１—４个核仁，核仁的大小随染色体的浓缩程度发生变化，在不同类型的细胞中，银染核仁的面积分布不同，说明ＲＮＡ与蛋白质的比例有颇大的变动。试验还证明，染色体标本的制备技术对银染的分化程度有较大的影响，只有在适当的条件下才能促使银染的正反应。
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| 关 键 词： | 硬粒豌豆，银染核仁，染色体分化 |
APPLICATION OF Ag-NOR DYEING TECHNIQUE ON PEA CHROMOSOME RESEARCH |
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| Li Guiquan et al..APPLICATION OF Ag-NOR DYEING TECHNIQUE ON PEA CHROMOSOME RESEARCH[J].Journal of Shanxi Agricultural University,1995(1). |
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| Authors: | Li Guiquan |
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| Abstract: | This paper studied pea cell nucleolus number, Ag-NOR seat changes and chromosome differentiation extent with hard-grain pea cultured in Shanxi. The results show that there were 1-4 nucleolus in interphase nucleus, its size exert changes as thechromosome concentrating extent. The distribution area of Ag-dyeing nucleolus wasdifferent in varying type cells, the results show that proportion of RNA and protein have produced a great changes. The experiment also proved that preparation technique of chromosome specimen exert a great influence on Ag-dyeing differentiation extent, and the positive reaction of Ag-dyeing can be promoted under suitable condition. |
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| Keywords: | Hard-grain per Ag-dyeing nucleolus Chromosome differentiation |
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