| 中国荷斯坦牛GlyCAM1基因遗传多态性的研究 |
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| 引用本文： | 李晶,王洪梅,王长法,李秋玲,侯明海,李建斌,柳楠,仲跻峰.中国荷斯坦牛GlyCAM1基因遗传多态性的研究[J].黑龙江畜牧兽医,2009(3). |
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| 作者姓名： | 李晶 王洪梅 王长法 李秋玲 侯明海 李建斌 柳楠 仲跻峰 |
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| 作者单位： | [1]青岛农业大学动物科技学院,山东青岛266109 [2]山东省农业科学院奶牛研究中心,山东济南250100 |
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| 基金项目： | 国家“863”计划项目(2006AA10Z1D9);;公益性行业科研专项项目(nyhyzx07-036-09);;山东省良种工程项目(2007LZ10-05) |
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| 摘 要： | 以273头中国荷斯坦牛为研究对象，利用CRS—PCR、PCR—SSCP及DNA测序技术检测了GlyCAM1基因外显子3、内含子3的遗传多态性。结果表明：GlyCAM1基因分别在外显子3和内含子3的第2081（A／C）、2417（C／T）位存在突变，2个位点的等位基因频率A／B分别为0．7525，0．2475和0．9046，0．0954；经菇。适合性检验，中国荷斯坦牛内含子3的突变达到Hardy—Weinberg平衡状态（P〉0．05），但其外显子3的突变未达到Hardy—Weinberg平衡状态（P〈0．05）。
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| 关 键 词： | 中国荷斯坦牛 糖基化依赖细胞黏附分子1 基因 遗传多态性 CRS—PCR PCR—SSCP |
Analysis of genetic polymorphisms at GlyCAM1 gene in China Holstein cattle |
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| LI Jing,Liu Nan,ZHONG Ji-feng,et al.Analysis of genetic polymorphisms at GlyCAM1 gene in China Holstein cattle[J].Heilongjiang Animal Science And veterinary Medicine,2009(3). |
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| Authors: | LI Jing Liu Nan ZHONG Ji-feng |
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| Institution: | 1.College of Animal Science & Technology;Qingdao Agricultural University;Qingdao 266109;China;2.Dairy Cattle Research Center;Shandong Academy of Agricultural Science;Jinan 250100;China |
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| Abstract: | The genetic polymorphism of GlyCAM1 gene exon 3 and intron 3 in China Holstein cattle were studied by CRS-PCR and PCR-SSCP and DNA sequencing.Sequencing results showed that two polymorphic sites were identified,which were at position 2 081(A/C) and 2 417(C/T) in the GlyCAM1 gene exon 3 and intron 3 respectively.In the 2 081 site the allelic frequencies of A and B was 0.752 5,0.247 5;In the 241 7 site the allelic frequencies of A and B was 0.904 6,0.095 4.x2 test indicated that the polymorphic sites of intro... |
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| Keywords: | China Holstein cattle GlyCAM1 gene genetic polymorphism CRS-PCR PCR-SSCP |
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