| 慈姑茎尖组织培养的研究（摘要） |
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| 引用本文： | 黄凯丰,时政.慈姑茎尖组织培养的研究（摘要）[J].农业科学与技术,2011,12(2):205-207. |
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| 作者姓名： | 黄凯丰 时政 |
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| 作者单位： | 贵州师范大学生命科学学院植物遗传育种研究所,贵州贵阳,550001 |
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| 基金项目： | 贵州省科学技术基金项目，贵阳市科技局农业攻关项目，贵州师范大学博士科研基金项目(2008年).Supported by Science and Technology Foundation of Guizhou Province，Key Agricultural Program from Science and Technology Bureau of Guiyang City，Science Research Program for Doctors in Guizhou Normal University |
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| 摘 要： | 目的]筛选出慈姑茎尖组织培养的最佳试验条件。方法]以江苏省慈姑的主栽品种＂宝应紫圆＂的茎尖为材料,研究了不同培养条件对慈姑茎尖培养和匍匐茎诱导的影响。结果]0.1mg/L6-BA＋0.05mg/LNAA培养条件下的成苗效果较好,2.00mg/L6-BA＋0.50mg/LNAA培养条件对慈姑匍匐茎的诱导效果较好;不同蔗糖浓度处理间对慈姑抽生匍匐茎的影响差异不显著。结论]不同培养条件对慈姑的成苗和匍匐茎诱导有一定影响。
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| 关 键 词： | 慈姑 组织培养 茎尖 |
A Preliminary Study on the Tissue Culture of Sagittaria trifolia L. |
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| HUANG Kai-feng,SHI Zheng.A Preliminary Study on the Tissue Culture of Sagittaria trifolia L.[J].Agricultural Science ＆ Technology,2011,12(2):205-207. |
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| Authors: | HUANG Kai-feng SHI Zheng |
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| Institution: | Institute of Plant Genetics and Breeding,School of Life Sciences,Guizhou Normal University,Guiyang 550001 |
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| Abstract: | Objective] This study was to optimize the experimental conditions for large scale propagation of Sagittaria trifolia L via tissue culture.Method] The dominant S.trifolia cultivar Baoyingziyuan introduced from Jiangsu Province was used as experimental material to study the impacts of various culture conditions on tissue culture of its stem tips and induction of stolons.Result] Hormone combination 0.10 mg/L 6-BA +0.05 mg/L NAA performed best in plantlet regeneration and 2.0 mg/L 6-BA +0.5 mg/L NAA best in induction of stolon.Various sucrose concentrations did not show significant difference in the impact on sprouted stolons.Conclusion] Various culture conditions could to some extent impact plantlet regeneration and stolon induction,and our results reveal the optimal hormone combinations for regeneration and stolon induction of S.trifolia. |
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| Keywords: | Sagittaria trifolia L Tissue culture Stem tip |
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