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大蒜根系分泌物对石榴枯萎病菌和枯草芽孢杆菌作用初探(摘要)
引用本文:汤东生,王斌,毛忠顺,何霞红.大蒜根系分泌物对石榴枯萎病菌和枯草芽孢杆菌作用初探(摘要)[J].农业科学与技术,2011,12(2):237-240.
作者姓名:汤东生  王斌  毛忠顺  何霞红
作者单位:云南农业大学生物多样性与病虫害控制教育部重点实验室,云南昆明,650201
基金项目:国家"973"项目(2011CB100400).Supported by National "973" Project
摘    要:目的]研究大蒜套种石榴控制石榴枯萎病的可能性。方法]在实验室条件下,采用不同浓度的0.2倍MS培养液和蒸馏水培养的大蒜根系分泌物,测定其对枯萎病菌和枯草芽孢杆菌生长的影响。结果]MS培养液和蒸馏水培养的大蒜根系分泌物均不能有效抑制枯萎病菌菌丝的生长。而同样2种方式培养的大蒜根系分泌物却对枯草芽孢杆菌的增殖具有较强的促进作用,且蒸馏水培养大蒜根系分泌物效果要优于MS培养液培养的根系分泌物。结论]石榴园套种大蒜结合枯草芽孢杆菌施用有望作为控制石榴枯萎病蔓延的一种方式。

关 键 词:大蒜  石榴枯萎病  甘薯长喙壳  枯草芽孢杆菌  根系分泌物

Preliminary Study on Garlic Root Exudates Influences to the Growth of Pomegranate Wilt Pathogen ( Ceratocystis fimbriata) and Bacillus subtilis
TANG Dong-sheng,WANG Bin,MAO Zhong-shun,HE Xia-hong.Preliminary Study on Garlic Root Exudates Influences to the Growth of Pomegranate Wilt Pathogen ( Ceratocystis fimbriata) and Bacillus subtilis[J].Agricultural Science & Technology,2011,12(2):237-240.
Authors:TANG Dong-sheng  WANG Bin  MAO Zhong-shun  HE Xia-hong
Institution:Key Laboratory for Agro-biodiversity and Pest control of Ministry of Education,Yunnan Agricultural University,Kunming 650201
Abstract:Objectives] This paper aims to explore the possibility to intercrop garlic with pomegranate tree to control pomegranate wilt.Methods] Root exudates of garlic is cultivated in 1/5 concentration of MS solution and distilled water is examined in lab to test their effect to growth of mycelia of pomegranate wilt pathogen(Ceratocystis fimbriata)and multiplication of Bacillus subtilis.Results] The result shows that garlic root exudates whatever cultivated in MS solution or distilled water could not inhibit or promote mycelia growth of C.fimbriata.However,garlic root exudates cultivated in both methods effectively promote multiplication of B.subtilis.Conclusions] It is suggested that intercropping garlic with pomegranate tree by combining application B.subtilis could be a promising way to prevent pomegranate wilt spread in practice.
Keywords:Garlic  Pomegranate wilt  Ceratocystis fimbriata  Bacillus subtilis  Root exudates
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